Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/11075
Title: High‑affinity transport, cyanide‑resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus.
Authors: Cunha, Aureliano Claret da
Gomes, Lorena Soares
Santos, Fernanda Godoy
Oliveira, Fábio Faria
Teixeira, Janaina Aparecida
Sampaio, Geraldo Magela Santos
Trópia, Maria José Magalhães
Castro, Ieso de Miranda
Lucas, Cândida Manuel Ribeiro Simões
Brandão, Rogélio Lopes
Keywords: Glycerol metabolism
Cachaça
STL1
Biotechnological applications
Issue Date: 2019
Citation: CUNHA, A. C. da et al. High‑affinity transport, cyanide‑resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus. Journal of Industrial Microbiology & Biotechnology, v. 2019, p. 1-15, 2019. Disponível em: <https://link.springer.com/article/10.1007%2Fs10295-018-02119-5>. Acesso em: 7 mar. 2019.
Abstract: Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaça (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
URI: http://www.repositorio.ufop.br/handle/123456789/11075
metadata.dc.identifier.uri2: https://link.springer.com/article/10.1007%2Fs10295-018-02119-5
ISSN: 1476-5535
Appears in Collections:DEFAR - Artigos publicados em periódicos

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