Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/11020
Title: In vitro antiplasmodial activity and identification, using tandem LC-MS, of alkaloids from Aspidosperma excelsum, a plant used to treat malaria in Amazonia.
Authors: Nascimento, Myrth Soares do
Pina, Nayla di Paula Vieira
Silva, Andressa Santa Brigida da
Gomes, Luís Fabio dos Santos
Vasconcellos, Flávio de
Brandão, Geraldo Célio
Nascimento, Maria Fernanda Alves do
Oliveira, Alaide Braga de
Barbosa, Wagner Luiz Ramos
Keywords: Apocynaceae
Anti-malarial activity
Indole-alkaloids
Plasmodium falciparum
Issue Date: 2019
Citation: NASCIMENTO, M. S. do et al. In vitro antiplasmodial activity and identification, using tandem LC-MS, of alkaloids from Aspidosperma excelsum, a plant used to treat malaria in Amazonia. Journal of Ethnopharmacology, v. 228, p. 99-109, jan. 2019. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0378874118316015?via%3Dihub>. Acesso em: 25 fev. 2019.
Abstract: Ethnopharmacological relevance: Aspidosperma excelsum Benth. (Apocynaceae), a native tree in the Brazilian Amazonia, is traditionally used to treat various diseases, including malaria. Aim of study: To investigate the chemical constitution, antiplasmodial activity and cytotoxicity of samples obtained from A. excelsum trunk bark by different procedures aiming to evaluate their potential as an antimalarial phytomedicine. Materials and methods: A hydroethanolic extract and alkaloid extracts were prepared and assayed for antiplasmodial activity and cytotoxicity against chloroquine-resistant Plasmodium falciparum (W2 strain) and HepG2 cells, respectively. Taking into account the known occurrence and antimalarial activity of Aspidosperma monoterpene indole alkaloids (MIA), acid-base extractions were carried out and the fractions were assayed for antiplasmodial activity and cytotoxicity. All the samples were analysed by hyphenated chromatographic techniques, such as UPLC-DAD-ESI-MS/MS and HRMS (HPLC-MS MicroTOF), comparing their chemical composition to the literature data. Results: The hydroethanolic extract disclosed a moderate in vitro activity against chloroquine-resistant Plasmodium falciparum (W2 strain) with IC50 23.68 ± 3.08 µg/mL), low cytotoxicity to HepG2 cells (> 250 µg/ mL) and good SI (> 10.56). A total of 20 known monoterpene indole alkaloids were identified, seven of which are here firstly described for A. excelsum. Known highly active alkaloids, namely demethylaspidospermine, aspidocarpine, and ochrolifuanine are present in active alkaloid fractions and might contribute to their observed antiplasmodial effect. An alkaloid fraction (Ae-Alk2), obtained directly from trunk bark by extraction with dil. aqueous HCl, pointed out for its activity (IC50 8.75 ± 2.26 µg/mL, CC50 185.14 ± 1.97 µg/mL, SI 21.16) and should be highlighted as the most promising out of the assayed samples. Conclusion: The present results represent a preliminary support to the alleged antimalarial use of A. excelsum trunk bark and allowed to highlight alkaloid fractions as promising phytomedicines.
URI: http://www.repositorio.ufop.br/handle/123456789/11020
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S0378874118316015
ISSN: 03788741
Appears in Collections:DEFAR - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_VitroAntiplasmodialActivity.pdf1,41 MBAdobe PDFView/Open    Request a copy


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.