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Title: Effect of pyridostigmine on in vivo and in vitro respiratory muscle of mdx mice.
Authors: Amancio, Gabriela de Cássia Sousa
Guimarães, Andrea Grabe
Haikel, Dridi
Moreau, Johan
Barcellos, Neila Marcia Silva
Lacampagne, Alain
Matecki, Stefan
Cazorla, Olivier
Keywords: Duchenne muscular dystrophy
Pyridostigmine bromide
Respiratory function
Diaphragm contractility
Issue Date: 2017
Citation: AMANCIO, D. de C. S. et al. Effect of pyridostigmine on in vivo and in vitro respiratory muscle of mdx mice. Respiratory Physiology & Neurobiology, v. 243, p. 107-114, set. 2017. Disponível em: <>. Acesso em: 25 fev. 2019.
Abstract: The current work was conducted to verify the contribution of neuromuscular transmission defects at the neuromuscular junction to Duchenne Muscular Dystrophy disease progression and respiratory dysfunction. We tested pyridostigmine and pyridostigmine encapsulated in liposomes (liposomal PYR), an acetylcholinesterase inhibitor to improve muscular contraction on respiratory muscle function in mdx mice at different ages. We evaluated in vivo with the whole-body plethysmography, the ventilatory response to hypercapnia, and measured in vitro diaphragm strength in each group. Compared to C57BL10 mice, only 17 and 22 month-old mdx presented blunted ventilatory response, under normocapnia and hypercapnia. Free pyridostigmine (1 mg/kg) was toxic to mdx mice, unlike liposomal PYR, which did not show any side effect, confirming that the encapsulation in liposomes is effective in reducing the toxic effects of this drug. Treatment with liposomal PYR, either acute or chronic, did not show any beneficial effect on respiratory function of this DMD experimental model. The encapsulation in liposomes is effective to abolish toxic effects of drugs.
ISSN: 15699048
Appears in Collections:DEFAR - Artigos publicados em periódicos

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