Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/10911
Title: Vaccination with a CD4+ and CD8+ T-cell epitopes-based recombinant chimeric protein derived from Leishmania infantum proteins confers protective immunity against visceral leishmaniasis.
Authors: Dias, Daniel Silva
Ribeiro, Patrícia Aparecida Fernandes
Martins, Vivian Tamietti
Lage, Daniela Pagliara
Costa, Lourena Emanuele
Chávez Fumagalli, Miguel Angel
Ramos, Fernanda Fonseca
Santos, Thaís Teodoro de Oliveira
Ribeiro, Fernanda Ludolf
Oliveira, Jamil Silvano de
Mendes, Tiago Antônio de Oliveira
Silva, Eduardo Sergio da
Galdino, Alexsandro Sobreira
Duarte, Mariana Costa
Roatt, Bruno Mendes
Souza, Daniel Menezes
Teixeira Junior, Antonio Lucio
Coelho, Eduardo Antônio Ferraz
Issue Date: 2018
Citation: DIAS, D. S. et al. Vaccination with a CD4+ and CD8+ T-cell epitopes-based recombinant chimeric protein derived from Leishmania infantum proteins confers protective immunity against visceral leishmaniasis. Translational Research, v. 200, p. 18-34, out. 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1931524418300847?via%3Dihub>. Acesso em: 22 fev. 2019.
Abstract: Vaccination seems to be the best approach to control visceral leishmaniasis (VL). Resistance against infection is based on the development of a Th1 immune response characterized by the production of interferons-γ (IFN-γ), interleukin-12 (IL-12), granulocyte-macrophage-colony-stimulating factor (GM-CSF), and tumor necrosis factor-α (TNF-α), among others. A number of antigens have been tested as potential targets against the disease; few of them are able to stimulate human immune cells. In the present study, 1 prediction of MHC class I and II molecules-specific epitopes in the amino acid sequences of 3 Leishmania proteins: 1 hypothetical, prohibitin, and small glutamine-rich tetratricopeptide repeat-containing proteins, was performed using bioinformatics tools, and a T-cell epitopes-based recombinant chimeric protein was constructed, synthetized and purified to be evaluated in invitro and in vivo experiments. The purified protein was tested regarding its immunogenicity in peripheral blood mononuclear cells (PBMCs) from healthy subjects and VL patients, as well as to its immunogenicity and protective efficacy in a murine model against Leishmania infantum infection. Results showed a Th1 response based on high IFN-γ and low IL-10 levels derived from in chimera-stimulated PBMCs in both healthy subjects and VL patients. In addition, chimera and/or saponin-immunized mice presented significantly lower parasite burden in distinct evaluated organs, when compared to the controls, besides higher levels of IFN-γ, IL-2, IL-12, and GM-CSF, and an IgG2a isotype-based humoral response. In addition, the CD4+ and CD8+ T-cell subtypes contributed to IFN-γ production in the protected animals. The results showed the immunogenicity in human cells and the protective efficacy against L. infantum in a murine model, and well indicate that this recombinant chimera can be considered as a promising strategy to be used against human disease.
URI: http://www.repositorio.ufop.br/handle/123456789/10911
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S1931524418300847
ISSN: 00222143
Appears in Collections:DECBI - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_VaccinationCD4CD8.pdf2,64 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.