Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/10909
Title: High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection.
Authors: Carvalho, Gerusa Brandão de
Costa, Lourena Emanuele
Lage, Daniela Pagliara
Ramos, Fernanda Fonseca
Santos, Thaís Teodoro de Oliveira
Ribeiro, Patrícia Aparecida Fernandes
Dias, Daniel Silva
Salles, Beatriz Cristina Silveira
Lima, Mariana Pedrosa
Carvalho, Lívia Mendes
Dias, Ana C. S.
Alves, Patrícia Terra
Franklin, Michelle Lucrécio
Silva, Renata A. M.
Duarte, Mariana Costa
Souza, Daniel Menezes
Roatt, Bruno Mendes
Chávez Fumagalli, Miguel Angel
Goulart Filho, Luiz Ricardo
Teixeira Junior, Antonio Lucio
Coelho, Eduardo Antônio Ferraz
Keywords: Immune response
Phage display
Peripheral blood mononuclear cells
Issue Date: 2019
Citation: CARVALHO, G. B. de et al. High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection. Parasitology, v. 146, n. 3, p. 322-332, mar. 2019. Disponível em: <https://www.cambridge.org/core/journals/parasitology/article/highthrough-identification-of-t-cellspecific-phageexposed-mimotopes-using-pbmcs-from-tegumentary-leishmaniasis-patients-and-their-use-as-vaccine-candidates-against-leishmania-amazonensis-infection/71AD5ADD21870A5C7D684E0E6D512E9D>. Acesso em: 22 fev. 2019.
Abstract: In the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera of TL patients and healthy subjects, besides the immune stimulation of peripheral blood mononuclear cells (PBMCs) collected from untreated and treated TL patients and healthy subjects. The clones were evaluated regarding their specific interferon-γ (IFN-γ) and interleukin-4 (IL-4) production in the in vitro cultures, and selectivity and specificity values were calculated, and those presenting the best results were selected for the in vivo experiments. Two clones, namely A4 and A8, were identified and used in immunization protocols from BALB/c mice to protect against Leishmania amazonensis infection. Results showed a polarized Th1 response generated after vaccination, being based on significantly higher levels of IFN-γ, IL-2, IL-12, tumour necrosis factor-α (TNF-α) and granulocyte-macrophage colony-stimulating factor (GM-CSF); which were associated with lower production of specific IL-4, IL-10 and immunoglobulin G1 (IgG1) antibodies. Vaccinated mice presented significant reductions in the parasite load in the infected tissue and distinct organs, when compared with controls. In conclusion, we presented a strategy to identify new mimotopes able to induce Th1 response in PBMCs from TL patients and healthy subjects, and that were successfully used to protect against L. amazonensis infection
URI: http://www.repositorio.ufop.br/handle/123456789/10909
metadata.dc.identifier.uri2: https://www.cambridge.org/core/journals/parasitology/article/highthrough-identification-of-t-cellspecific-phageexposed-mimotopes-using-pbmcs-from-tegumentary-leishmaniasis-patients-and-their-use-as-vaccine-candidates-against-leishmania-amazonensis-infection/71AD5ADD21870A5C7D684E0E6D512E9D
ISSN: 00311820
Appears in Collections:DECBI - Artigos publicados em periódicos

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