Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/10875
Title: Resveratrol acts anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis by modulating the hippocampal miRNome.
Authors: Queiroz, Karina Barbosa de
Pereira, Thaís dos Santos Fontes
Araújo, Márcio Sobreira Silva
Gomez, Ricardo Santiago
Coimbra, Roney Santos
Keywords: Streptococcus pneumoniae
Neuroprotection
microRNA
Issue Date: 2018
Citation: QUEIROZ, K. B. de et al. Resveratrol acts anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis by modulating the hippocampal miRNome. Molecular Neurobiology, v. 55, n. 12, p. 8869-8884, dez. 2018. Disponível em: <https://link.springer.com/article/10.1007%2Fs12035-018-1037-5>. Acesso em: 21 fev. 2019.
Abstract: Resveratrol (RSV) is anti-inflammatory and neuroprotective, cross the blood–brain barrier (BBB) and has a safe profile. Besides, RSV modulates the expression of some miRNAs related to neurological disorders. Thus, we hypothesized that RSV can be neuroprotective in pneumococcal meningitis by modulating the global microRNA expression profile (miRNome). Eleven-day old rats were intracysternally infected with S. pneumoniae (~ 2 × 106 c.f.u.) and were orally administered with RSV (50 mg/kg) or vehicle in pre-treatment (before infection) or post-treatment schedules (3 and 18 h p.i.). At 24 h p.i., animals were euthanized and apoptotic cells were counted in the hippocampal dentate gyrus of the right brain hemispheres. The hippocampi from left hemispheres were used for cytokines and chemokines multiplex assay and miRNome profiling with TaqMan OpenArray Rodent MicroRNA. Infected rats treated with RSV had lower apoptotic scores and IL-1β, CCL2, and CCL3 levels when compared to the infected group receiving placebo. Seven miRNAs were down regulated, and 18 were up regulated by pneumococcal acute meningitis. Thirty-seven miRNAs were down regulated, and three were up regulated (hsa-miR-15b-5p, hsa-miR-25-3p, hsa-miR-125b-5p) by the interaction between meningitis and RSV. Pathway enriched analysis revealed that meningitis and RSV modulate the expression of miRNAs targeting critical pathways related to the pathophysiology of bacterial meningitis. Nevertheless, hsa-miR-25-3p and hsa-miR-125b-5p target the transcription factor TEF-1, for which there are binding sites in Il-1β, Ccl2, and Ccl3 genes. RSV is anti-inflammatory and neuroprotective in an infant rat model of pneumococcal meningitis and these positive effects involve the modulation of the hippocampal miRNome.
URI: http://www.repositorio.ufop.br/handle/123456789/10875
metadata.dc.identifier.uri2: https://link.springer.com/article/10.1007%2Fs12035-018-1037-5
ISSN: 15591182
Appears in Collections:DEALI - Artigos publicados em periódicos

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