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Title: Evaluation of parasite and host genetics in two generations of a family with Chagas disease.
Authors: Lima, Ana Paula Braga
Oliveira, Maykon Tavares de
Silva, Rafael Rodrigues
Torres, Rosália Morais
Veloso, Vanja Maria
Lana, Marta de
Silva, Glenda Nicioli da
Keywords: Host genetic polymorphism
Chagas disease evolution
Trypanosoma cruzi genetics
Issue Date: 2018
Citation: LIMA, A. P. B. et al. Evaluation of parasite and host genetics in two generations of a family with Chagas disease. Parasitology Research, v. 117, n. 9, p. 3009-3013, set. 2018. Disponível em: <>. Acesso em: 21 fev. 2019.
Abstract: Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered to be a multifactorial disease associated with host and parasite genetics, which influence clinical aspects of the disease and other host conditions. In order to understand better the evolution of the disease, this study intended to evaluation of parasite and host genetics in two generations of a family with Chagas disease from the Alto Paranaiba region, Minas Gerais, Brazil, comprising a mother and her five daughters. Several features were evaluated, including the characterization of T. cruzi directly from the blood of patients, host polymorphisms of genes related to cardiomyopathy (TNF, WISP1, CCR5, and TGF-β1) and clinical aspects of the patients. To verify the intraspecific variability of the parasite, the characterization was done directly from human blood using the PCR-LSSP technique and analyzed based on Dice coefficient and unweighted pair group analysis (UPGMA). The host polymorphism was evaluated by PCR-RFLP. The global results showed low variability of the parasites characterized from blood of patients, through Shannon index (0.492) and mean heterozygosity value per locus (0.322). All six patients presented the same genetic polymorphism profile for TNF, WISP1, and TGF-β1, and only one patient was homozygous to CCR5, which suggests that there is no association between the clinical aspects of the patients and their genetic profiles. In conclusion, the findings confirm that the understanding of the clinical evolution of Chagas disease goes beyond the genetic aspects of the parasite and the host.
ISSN: 14321955
Appears in Collections:DEACL - Artigos publicados em periódicos

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