Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/10836
Title: Mixed formulation of conventional and pegylated meglumine antimoniate-containing liposomes reduces inflammatory process and parasite burden in Leishmania infantum-infected BALB/c mice.
Authors: Reis, Levi Eduardo Soares
Brito, Rory Cristiane Fortes de
Cardoso, Jamille Mirelle de Oliveira
Mathias, Fernando Augusto Siqueira
Soares, Rodrigo Dian de Oliveira Aguiar
Carneiro, Cláudia Martins
Vieira, Paula Melo de Abreu
Ramos, Guilherme Santos
Frezard, Frederic Jean Georges
Roatt, Bruno Mendes
Reis, Alexandre Barbosa
Keywords: Chemotherapy
Visceral leishmaniasis
Issue Date: 2017
Citation: REIS, L. E. S. et al. Mixed formulation of conventional and pegylated meglumine antimoniate-containing liposomes reduces inflammatory process and parasite burden in Leishmania infantum-infected BALB/c mice. Antimicrobial Agents and Chemotherapy, v. 61, n. 11, p. 1-13, nov. 2017. Disponível em: <https://aac.asm.org/content/61/11/e00962-17.long>. Acesso em: 21 fev. 2019.
Abstract: Pentavalent antimonial has been the first choice treatment for visceral leishmaniasis; however, it has several side effects that leads to low adherence to treatment. Liposome-encapsulated meglumine antimoniate (MA) arises as an important strategy for chemotherapy enhancement. We evaluated the immunopathological changes using the mixture of conventional and pegylated liposomes with MA. The mice were infected with Leishmania infantum and a single-dose treatment regimen. Comparison was made with groups treated with saline, empty liposomes, free MA, and a liposomal formulation of MA (Lipo MA). Histopathological analyses demonstrated that animals treated with Lipo MA showed a significant decrease in the inflammatory process and the absence of granulomas. The in vitro stimulation of splenocytes showed a significant increase of gamma interferon (IFN-γ) produced by CD8+ T cells and a decrease in interleukin-10 (IL-10) produced by CD4+ and CD8+ T cells in the Lipo MA. Furthermore, the Lipo MA group showed an increase in the IFN-γ/IL-10 ratio in both CD4+ and CD8+ T cell subsets. According to the parasite load evaluation using quantitative PCR, the Lipo MA group showed no L. infantum DNA in the spleen (0.0%) and 41.4% in the liver. In addition, we detected a low positive correlation between parasitism and histopathology findings (inflammatory process and granuloma formation). Thus, our results confirmed that Lipo MA is a promising antileishmanial formulation able to reduce the inflammatory response and induce a type 1 immune response, accompanied by a significant reduction of the parasite burden into hepatic and splenic compartments in treated animals.
URI: http://www.repositorio.ufop.br/handle/123456789/10836
metadata.dc.identifier.uri2: https://aac.asm.org/content/61/11/e00962-17.long
ISSN: 10986596
Appears in Collections:DEACL - Artigos publicados em periódicos

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