Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1065
Title: Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis.
Authors: Rosário, Eliza Yoshie do
Genaro, Odair
Silva, João Carlos França da
Costa, Roberto Teodoro da
Mayrink, Wilson
Reis, Alexandre Barbosa
Carneiro, Mariângela
Keywords: Canine visceral leishmaniasis
Leishmania chagasi
Diagnosis
Recombinant antigens
Issue Date: 2005
Citation: ROSÁRIO, E. Y. do et al. Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis. Memórias do Instituto Oswaldo Cruz, v. 100, n. 2, p. 197-203, 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n2/v100n2a15.pdf>. Acesso em: 10 jul. 2012
Abstract: The performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 1:40 titre, 106 (92.2%) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 1:40 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100%). Specificity was higher than 96% for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88%). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97). Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however.
URI: http://www.repositorio.ufop.br/handle/123456789/1065
ISSN: 16788060
metadata.dc.rights.license: O periódico, Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto deposite uma cópia eletrônica dos artigos publicados por esse periódico em que ao menos um dos autores faça parte da comunidade cientifica da Universidade Federal de Ouro Preto. Fonte: Licença concedida por e-mail em 12 dez. 2013.
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