Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/1056
Title: Isotype patterns of immunoglobulins : hallmarks for clinical status and tissue parasite density in brazilian dogs naturally infected by Leishmania (Leishmania) chagasi.
Authors: Reis, Alexandre Barbosa
Carvalho, Andréa Teixeira de
Vale, André Macedo
Marques, Marcos José
Giunchetti, Rodolfo Cordeiro
Mayrink, Wilson
Guerra, Luanda Liboreiro
Andrade, Renata Aline de
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
Keywords: Canine visceral leishmaniasis
Clinical forms
Tissue parasitism
Immunoglobulin profile
Leishmania chagasi
Issue Date: 2006
Citation: REIS, A. B. et al. Isotype patterns of immunoglobulins : hallmarks for clinical status and tissue parasite density in brazilian dogs naturally infected by Leishmania (Leishmania) chagasi. Veterinary Immunology and Immunopathology, v. 112, n. 3-4, p. 102-116, ago. 2006. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242706000444>. Acesso em: 10 jul. 2010.
Abstract: The role of anti-leishmanial immune response underlying the susceptibility/resistance during canine visceral leishmaniasis (CVL) has been recognized throughout ex vivo and in vitro investigations. Recently, we demonstrated that immunoglobulin levels (Igs), as well as the parasite load are relevant hallmarks of distinct clinical status of CVL. To further characterize and upgrade the background on this issue, herein, we have evaluated, inLeishmania ( Leishmania ) chagasinaturally infected dogs, the relationship between tissue parasitism (skin, bone marrow, spleen, liver and lymph node), the CVL clinical status (asymptomatic (AD), with no suggestive signs of the disease; oligosymptomatic (OD), with maximum three clinical signs—opaque bristles; localized alopecia and moderate loss of weight; symptomatic (SD), serologically positive with severe clinical signs of visceral leishmaniasis), and the humoral immunological profile of anti-Leishmania immunoglobulins (IgG, IgG1, IgG2, IgM, IgA and IgE). Our major statistically significant findings revealed distinct patterns of tissue parasite density within L. chagasi-infected dogs despite their clinical status, pointing out the spleen and skin as the most relevant sites of high parasitism during ongoing CVL. Parasite density of bone marrow and spleen were the most reliable parasitological markers to decode the clinical status of CVL. Moreover, the parasite density of bone marrow better correlates with most anti- Leishmania Igs reactivity. Additionally, a prognostic hallmark for canine visceral leishmaniasis was found, highlighting strong correlation between IgG1 and asymptomatic disease, but with IgA, IgE and IgG2 displaying better association with symptomatic disease. The new aspects of this study highlighted pioneer findings that correlated the degree of tissue parasite density (low (LP), medium (MP) and high (HP) parasitism) with distinct patterns of anti- Leishmania Igs reactivity. In this scope, our data re-enforce the anti- Leishmania IgG but with IgA reactivity as the better marker for overall tissue parasitism. The association between clinical status, Ig profile and the tissue parasitism support a novel investigation on the impact of humoral immune response and susceptibility/resistance mechanism during ongoing CVL
URI: http://www.repositorio.ufop.br/handle/123456789/1056
ISSN: 01652427
metadata.dc.rights.license: O periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3291410158044.
Appears in Collections:DEACL - Artigos publicados em periódicos

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