Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/handle/123456789/10377
Title: Cytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes.
Authors: Santos, Edjane Rocha dos
Graminha, Angelica Ellen
Schultz, Mario Sergio
Correia, Isabel
Araujo, Heloisa Sobreiro Selistre de
Correa, Rodrigo de Souza
Ellena, Javier Alcides
Lacerda, Elisângela de Paula Silveira
Pessoa, João Costa
Batista, Alzir Azevedo
Keywords: Ruthenium complexes
Human serum albumin
Circular dichroism
Issue Date: 2018
Citation: SANTOS, E. R. dos et al. Cytotoxic activity and structural features of Ru(II)/phosphine/amino acid complexes. Journal of Inorganic Biochemistry, v. 182, p. 48-60, mai. 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S016201341730510X>. Acesso em: 05 abr. 2018.
Abstract: Thirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of α-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P = 1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N = 4,4′-dimethyl-2,2′-bipyridine (4′-Mebipy), 5,5′-dimethyl-2,2′-bipyridine (5′-Mebipy) or 4,4′-Methoxy-2-2′-bipyridine (4′-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA = glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met). All compounds were characterized by elemental analysis, spectroscopic and electrochemical techniques. The [Ru(AA-H)(P-P)(N-N)]PF6 complexes are octahedral (the AA-H ligand binding involves N-amine and O-carboxylate), diamagnetic (low-spin d6, S = 0) and present bands due to electronic transitions in the visible region. 1H, 13C{1H} and 31P{1H} NMR spectra of the complexes indicate the presence of C2 symmetry, and the identification of diastereoisomers. In vitro cytotoxicity assays of the compounds and cisplatin were carried out using MDA-MB-231 (human breast) tumor cell line and a non-tumor breast cell line (MCF-10A). Most complexes present promising results with IC50 values comparable with the reference drug cisplatin and high selectivity indexes were found for the complexes containing L-Trp. The binding of two Ru-precursors of the type [RuCl2(dppb)(NN)] (N-N = 4′-MeObipy or 4′-Mebipy) to the blood transporter protein human serum albumin (HSA) was evaluated by fluorescence and circular dichroism spectroscopy. Both complexes bind HSA, probably in the hydrophobic pocket near Trp214, and the Ru-complex containing 4′-MeObipy shows higher affinity for HSA than the 4′-Mebipy one.
URI: http://www.repositorio.ufop.br/handle/123456789/10377
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S016201341730510X#!
ISSN: 01620134
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