Navegando por Autor "Santiago, Andrezza Fernanda"
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Item Ageing down-modulates liver inflammatory immune responses to schistosome infection in mice.(2010) Faria, Elaine Speziali de; Aranha, Claudio Henrique Magalhães; Carvalho, Andréa Teixeira de; Santiago, Andrezza Fernanda; Oliveira, Rafael Pires de; Rezende, Michelle Carvalho de; Carneiro, Cláudia Martins; Corrêa, Deborah Aparecida Negrão; Coelho, Paulo Marcos Zech; Faria, Ana Maria Caetano deAgeing is associated with several alterations in the immune system. Our aim in this study was to compare the development of immunity to Schistosoma mansoni infection in young versus aged C57Bl ⁄ 6 mice using the liver as the main organ to evaluate pathological alterations and immune responses. In the acute phase, young mice had large liver granulomas with fibrosis and inflammatory cells. Chronic phase in young animals was associated with immunomodulation of granulomas that became reduced in size and cellular infiltrate. On the other hand, aged animals presented granulomas of smaller sizes already in the acute phase. Chronic infection in these mice was followed by no alteration in any of the inflammatory parameters in the liver. In concert with this finding, there was an increase in activated CD4+ T, CD19+ B and NK liver cells in young mice after infection whereas old mice had already higher frequencies of activated B, NK and CD4+ T liver cells and infection does not change these frequencies. After infection, liver production of inflammatory and regulatory cytokines such as IFN-c, IL-4 and IL-10 increased in young but not in old mice that had high levels of IL-4 and IL-10 regardless of their infection status. Our data suggest that the unspecific activation status of the immune system in aged mice impairs inflammatory as well as regulatory immune responses to S. mansoni infection in the liver, where major pathological alterations and immunity are at stage. This poor immune reactivity may have a beneficial impact on disease development.Item Consumption of conjugated linoleic acid (CLA)-supplemented diet during colitis development ameliorates gut inflammation without causing steatosis in mice.(2018) Moreira, Thais Garcias; Santos, Ana Cristina Gomes; Horta, Laila Sampaio; Miranda, Mariana Camila Gonçalves; Santiago, Andrezza Fernanda; Gonçalves, Juliana Lauar; Reis, Daniela Silva dos; Castro Junior, Archimedes Barbosa de; Santos, Luísa Lemos dos; Guimarães, Mauro Andrade de Freitas; Aguilar, Edenil Costa; Pap, Attila; Amaral, Joana Ferreira do; Leite, Jacqueline Isaura Alvarez; Machado, Denise Carmona Cara; Rezende, Rafael Machado; Nagy, Laszlo; Faria, Ana Maria Caetano de; Maioli, Tatiani UceliDietary supplementation with conjugated linoleic acid (CLA) has been proposed for weight management and to prevent gut inflammation. However, some animal studies suggest that supplementation with CLA leads to the development of nonalcoholic fatty liver disease. The aims of this study were to test the efficiency of CLA in preventing dextran sulfate sodium (DSS)-induced colitis, to analyze the effects of CLA in the liver function, and to access putative liver alterations upon CLA supplementation during colitis. So, C57BL/6 mice were supplemented for 3 weeks with either control diet (AIN-G) or 1% CLA-supplemented diet. CLA content in the diet and in the liver of mice fed CLA containing diet were accessed by gas chromatography. On the first day of the third week of dietary treatment, mice received ad libitum a 1.5%–2.5% DSS solution for 7 days. Disease activity index score was evaluated; colon and liver samples were stained by hematoxylin and eosin for histopathology analysis and lamina propria cells were extracted to access the profile of innate cell infiltrate. Metabolic alterations before and after colitis induction were accessed by an open calorimetric circuit. Serum glucose, cholesterol, triglycerides and alanine aminotransaminase were measured; the content of fat in liver and feces was also accessed. CLA prevented weight loss, histopathologic and macroscopic signs of colitis, and inflammatory infiltration. Mice fed CLA-supplemented without colitis induction diet developed steatosis, which was prevented in mice with colitis probably due to the higher lipid consumption as energy during gut inflammation. This result suggests that CLA is safe for use during gut inflammation but not at steady-state conditions.Item Effect of a protein-free diet in the development of food allergy and oral tolerance in BALB/c mice.(2015) Silva, Josiely Paula; Santiago, Andrezza Fernanda; Oliveira, Rafael Pires de; Rosa, Magda Luciana Paula; Carvalho, Cláudia Rocha; Amaral, Joana Ferreira do; Faria, Ana Maria Caetano deThe aim of the present study was to investigate the effect of a protein-free diet in the induction of food allergy and oral tolerance in BALB/c mice. The experimental model used was mice that were fed, since weaning up to adulthood, a balanced diet in which all dietary proteins were replaced by amino acid diet (Aa). The absence of dietary proteins did not prevent the development of food allergy to ovalbumin (OVA) in these mice. However, Aa-fed mice produced lower levels of IgE, secretory IgA and cytokines. In addition, when compared with mice from control group, Aa-fed mice had a milder aversive reaction to the allergen measured by consumption of OVA-containing solution and weight loss during food allergy development. In addition, mice that did not have dietary proteins in their diets were less susceptible to induction of oral tolerance. One single oral administration was not enough to suppress specific serum Ig and IgG1 levels in the Aa-fed group, although it was efficient to induce suppression in the control group. The present results indicate that the stimulation by dietary proteins alters both inflammatory reactivity and regulatory immune reactivity in mice probably due to their effect in the maturation of the immune system.