Navegando por Autor "Salustiano, Iorrana Vieira"
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Item Antibacterial screening of plants from the Brazilian Atlantic Forest led to the identification of active compounds in Miconia latecrenata (DC.) Naudin.(2020) Rodrigues, Laís Azevedo; Almeida, Ayla das Chagas; Gontijo, Douglas da Costa; Salustiano, Iorrana Vieira; Almeida, Alisson Andrade; Brandão, Geraldo Célio; Ribon, Andrea de Oliveira Barros; Leite, João Paulo VianaAntibiotic resistance is a serious global threat to public health. This has promoted the research for new drug targets, and the use of other approaches, such as antimicrobial combined therapy. The present study evaluated the antibacterial activity of 88 extracts from Brazilian Atlantic Forest trees. The organic extract from leaves of Miconia latecrenata (EMl) was the most promising for inhibiting the growth of Staphylococcus aureus (0.3 mg/mL) and Pseudomonas aeruginosa (2.5 mg/mL). After the bioguided frac tionation of EMl and metabolite profiling performed by UPLC DAD-MS/MS, the ethyl acetate (AFMl) and aqueous (WFMl) frac tions showed a mixture of phenolic compounds derived from ellagic acid and quercetin. The MIC value of AFMl was two-times lower than EMl for P. aeruginosa, suggesting that these phenolic compounds can perform bioactivity. Furthermore, EMI and AFMl showed synergism with ampicillin and tetracycline for S. aureus and P. aeruginosa, respectively. These findings suggest that extract and fractions of the Miconia latecrenata leaves can be used as therapeutic antibacterial agents.Item Parasitological and immunological evaluation of a novel chemotherapeutic agent against visceral leishmaniasis.(2020) Pereira, Isabela Amorim Gonçalves; Mendonça, Débora Vasconcelos Costa; Tavares, Grasiele de Sousa Vieira; Lage, Daniela Pagliara; Ramos, Fernanda Fonseca; Silva, João Augusto Oliveira da; Antinarelli, Luciana Maria Ribeiro; Machado, Amanda Sanchez; Carvalho, Lívia Mendes; Carvalho, Ana Maria Ravena Severino; Salustiano, Iorrana Vieira; Reis, Thiago Alves Rosa dos; Bandeira, Raquel Soares; Silva, Alessandra M.; Martins, Vivian Tamietti; Chávez Fumagalli, Miguel Angel; Humbert, Maria Victoria; Roatt, Bruno Mendes; Duarte, Mariana Costa; Souza, Daniel Menezes; Coimbra, Elaine Soares; Leite, João Paulo Viana; Coelho, Eduardo Antônio Ferraz; Gonçalves, Denise UtschAims: Treatment for visceral leishmaniasis (VL) is hampered by the toxicity and/or high cost of drugs, as well as by emergence of parasite resistance. Therefore, there is an urgent need for new antileishmanial agents. Methods and Results: In this study, the antileishmanial activity of a diprenylated flavonoid called 5,7,3,4’-tetrahydroxy-6,8-diprenylisoflavone (CMt) was tested against Leishmania infantum and L amazonensis species. Results showed that CMt presented selectivity index (SI) of 70.0 and 165.0 against L infantum and L amazonensis promastigotes, respectively, and of 181.9 and 397.8 against respective axenic amastigotes. Amphotericin B (AmpB) showed lower SI values of 9.1 and 11.1 against L infantum and L amazonensis promastigotes, respectively, and of 12.5 and 14.3 against amastigotes, respectively. CMt was effective in the treatment of infected macrophages and caused alterations in the parasite mitochondria. L infantum-infected mice treated with miltefosine, CMt alone or incorporated in polymeric micelles (CMt/Mic) presented significant reductions in the parasite load in distinct organs, when compared to the control groups. An antileishmanial Th1-type cellular and humoral immune response were developed one and 15 days after treatment, with CMt/Mic-treated mice presenting a better protective response. Conclusion: Our data suggest that CMt/Mic could be evaluated as a chemotherapeutic agent against VL.