Navegando por Autor "Rabelo, Ana Carolina Silveira"
Agora exibindo 1 - 12 de 12
Resultados por página
Opções de Ordenação
Item Anti-Inflammatory and antioxidant properties of black mulberry (Morus nigra L.) in a model of LPS-induced sepsis.(2018) Lúcio, Karine de Pádua; Rabelo, Ana Carolina Silveira; Araújo, Carolina Morais; Brandão, Geraldo Célio; Souza, Gustavo Henrique Bianco de; Silva, Regislainy Gomes da; Souza, Débora Maria Soares de; Silva, André Talvani Pedrosa da; Bezerra, Frank Silva; Calsavara, Allan Jefferson Cruz; Costa, Daniela CaldeiraSepsis is a complex disease and is the cause of many deaths worldwide. Sepsis pathogenesis involves a dysregulated inflammatory response with consequent production of inflammatory mediators and reactive species. The production and excessive release of these substances into the systemic circulation trigger various cellular and metabolic alterations that are observed during the disease evolution. Thus, more studies have been carried out to investigate the therapeutic potential of plants such as Morus nigra L., popularly known as black mulberry. Studies have shown that plants belonging to the Morus genus are rich in secondary metabolites such as flavonoids which are associated with important biological activities as antioxidant and anti-inflammatory actions. Based on this context, the objective of our study was to evaluate the anti-inflammatory and antioxidant properties of Morus nigra L. in a sepsis model induced by LPS. Male C57BL/6 mice were distributed in four groups: control, sepsis, sepsis treated with leaf extract of mulberry, and sepsis treated with mulberry pulp. The animals were treated with 100 μL of their respective treatments for twenty-one days. Sepsis was induced at the 21st day with lipopolysaccharide (LPS) by intraperitoneal injection. The animals were euthanized 24 hours after receiving the LPS injection. The data obtained were analyzed in GraphPad Prism 6.0 software. Our results showed that treatment with either extract significantly decreased the number of leukocytes in the bronchoalveolar lavage fluid and serum levels of TNF in septic animals. Regarding the redox status, the treatments significantly decreased the antioxidant activity of the enzyme glutathione peroxidase. Regarding metalloproteinase type 2, it was observed that the treatment with black mulberry pulp was able to significantly reduce the activity of this enzyme concerning the sepsis group. Finally, these results together promoted an increase in the animal’s survival that received the black mulberry leaf or pulp extract.Item Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity.(2017) Rabelo, Ana Carolina Silveira; Araújo, Glaucy Rodrigues de; Lúcio, Karine de Pádua; Araújo, Carolina Morais; Miranda, Pedro Henrique de Amorim; Silva, Breno de Mello; Carneiro, Ana Cláudia Alvarenga; Ribeiro, Erica Milena de Castro; Lima, Wanderson Geraldo de; Souza, Gustavo Henrique Bianco de; Brandão, Geraldo Célio; Costa, Daniela CaldeiraThe metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH, besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous extract.Item Araçá (Psidium cattleianum Sabine) ameliorates liver damage and reduces hepatic steatosis in rats fed with a high-fat diet.(2019) Paulino, Alice Helena de Souza; Viana, Ana Maria Fernandes; Bonomo, Larissa de Freitas; Guerra, Joyce Ferreira da Costa; Lopes, Juliana Márcia Macedo; Rabelo, Ana Carolina Silveira; Fagundes, Miliane Martins de Andrade; Regis, Ana Lúcia Rissoni dos Santos; Lima, Wanderson Geraldo de; Pedrosa, Maria Lúcia; Silva, Marcelo EustáquioBioactive compounds, present in some foods, act enhancing the endogenous antioxidant system and are proposed as an effective strategy in preventing the changes induced by free radicals in some diseases, such as nonalcoholic fatty liver disease (NAFLD). There has been an increase in the number of studies carried out with the aim of finding natural antioxidant compounds present in fruits, mainly the native fruits of Brazil, because they contain a high content of these compounds. Araçá (Psidium cattleianum Sabine) is a fruit that is rich in polyphenols and exhibits strong antioxidant, antiproliferative, and anti-inflammatory activities. Therefore, the present study investigated the effects of araçá flour on oxidative stress, liver injury, and antioxidant defenses in high-fat diet-induced hepatic steatosis in rats. In vitro experiments showed that araçá contains high concentrations of total polyphenols and exhibits strong antioxidant activity with no cytotoxicity. In vivo experiments indicated that the addition of araçá to a high-fat diet inhibited the activities of alanine aminotransferase and aspartate enzymes, reduced macrovesicular steatosis, increased the paraoxonase activity, and increased the concentration of the total and reduced forms of glutathione. Therefore, our findings suggested the hepatoprotective role of araçá against the progression of steatosis.Item Baccharis trimera (Carqueja) Improves metabolic and redox status in an experimental model of type 1 diabetes.(2018) Kaut, Natália Nogueira do Nascimento; Rabelo, Ana Carolina Silveira; Araújo, Glaucy Rodrigues de; Taylor, Jason Guy; Silva, Marcelo Eustáquio; Pedrosa, Maria Lúcia; Chaves, Míriam Martins; Rossoni Júnior, Joamyr Victor; Costa, Daniela CaldeiraDiabetes mellitus is a metabolic disorder that causes severe complications due to the increased oxidative stress induced by disease. Many plants are popularly used in the treatment of diabetes, e.g., Baccharis trimera (carqueja). The aim of this study was to explore the potential application of the B. trimera hydroethanolic extract in preventing redox stress induced by diabetes and its hypoglycemic properties. Experiments were conducted with 48 female rats, divided into 6 groups, named C (control), C600 (control + extract 600 mg/kg), C1200 (control + extract 1200 mg/kg), D (diabetic), D600 (diabetic + 600 mg/kg), and D1200 (diabetic + 1200 mg/kg). Type 1 diabetes was induced with alloxan, and the animals presented hyperglycemia and reduction in insulin and body weight. After seven days of experimentation, the nontreated diabetic group showed changes in biochemical parameters (urea, triacylglycerol, alanine aminotransferase, and aspartate aminotransferase) and increased carbonyl protein levels. Regarding the antioxidant enzymes, an increase in superoxide dismutase activity was observed but in comparison a decrease in catalase and glutathione peroxidase activity was noted which suggests that diabetic rats suffered redox stress. In addition, the mRNA of superoxide dismutase, catalase, and glutathione peroxidase enzymes were altered. Treatment of diabetic rats with B. trimera extract resulted in an improved glycemic profile and liver function, decreased oxidative damage, and altered the expression of mRNA of the antioxidants enzymes. These results together suggest that B. trimera hydroethanolic extract has a protective effect against diabetes.Item Baccharis trimera (carqueja) promotes gastroprotection on ethanol-induced acute gastric ulcer.(2020) Rabelo, Ana Carolina Silveira; Camini, Fernanda Caetano; Bittencourt, Melissa Maia; Lacerda, Kíssyla Christine Duarte; Lima, Wanderson Geraldo de; Costa, Daniela CaldeiraOne of the factors that can promote gastric ulcer is the acute and/or chronic consumption of ethanol. One of the most used therapeutic measures is the use of proton pump inhibitor, like omeprazole. However, these drugs have several side efects, making it necessary to search for new alternatives. To investigate the gastroprotective efect of Baccharis trimera (carqueja) we used an ethanol-induced acute gastric ulcer model. For this, gastric ulcer was induced in rats using absolute ethanol for 2 consecutive days. After analysis of the stomach, an increase in oxidative stress was observed, as well as the reduction of the antioxidant defense. In addition, we observed in histology that there was an increase in infammatory cells, necrosis, hemorrhage and hyperemia. Treatment with B. trimera was able to decrease oxidative damage as well as improve antioxidant defense. There was also a decrease in infammation, collagen area and hemorrhage. All the benefcial efects promoted by carqueja were similar to those found by the positive controls used in our study (vitamin C and omeprazole). Analyzing our data, we can conclude that B. trimera presents a gastroprotective efect and should be further studied for its application in gastric ulcer therapy.Item Baccharis trimera inhibits reactive oxygen species production through PKC and down-regulation p47phox phosphorylation of NADPH oxidase in SK Hep-1 cells.(2017) Araújo, Glaucy Rodrigues de; Rabelo, Ana Carolina Silveira; Meira, Janaína Serenato; Rossoni Júnior, Joamyr Victor; Borges, William de Castro; Cota, Renata Guerra de Sá; Batista, Maurício Azevedo; Lemos, Denise da Silveira; Souza, Gustavo Henrique Bianco de; Brandão, Geraldo Célio; Chaves, Míriam Martins; Costa, Daniela CaldeiraBaccharis trimera, popularly known as ‘‘carqueja’’, is a native South-American plant possessing a high concentration of polyphenolic compounds and therefore high antioxidant potential. Despite the antioxidant potential described for B. trimera, there are no reports concerning the signaling pathways involved in this process. So, the aim of the present study was to assess the influence of B. trimera on the modulation of PKC signaling pathway and to characterize the effect of the nicotinamide adenine dinucleotide phosphate oxidase enzyme (NOX) on the generation of reactive oxygen species in SK Hep-1 cells. SK-Hep 1 cells were treated with B. trimera, quercetin, or rutin and then stimulated or notwith PMA/ionomycin and labeled with carboxy H2DCFDA for detection of reactive oxygen species by flow cytometer. The PKC expression by Western blot and enzyme activity was performed to evaluate the influence of B. trimera and quercetin on PKC signaling pathway. p47phox and p47phox phosphorylated expression was performed byWestern blot to evaluate the influence of B. trimera on p47phox phosphorylation. The results showed that cells stimulated with PMA/ionomycin (activators of PKC) showed significantly increased reactive oxygen species production, and this production returned to baseline levels after treatment with DPI (NOX inhibitor). Both B. trimera and quercetin modulated reactive oxygen species production through the inhibition of PKC protein expression and enzymatic activity, also with inhibition of p47phox phosphorylation. Taken together, these results suggest that B. trimera has a potentialmechanism for inhibiting reactive oxygen species production through the PKC signaling pathway and inhibition subunit p47phox phosphorylation of nicotinamide adenine dinucleotide phosphate oxidase.Item Baccharis trimera protects against ethanol induced hepatotoxicity in vitro and in vivo.(2018) Rabelo, Ana Carolina Silveira; Lúcio, Karine de Pádua; Araújo, Carolina Morais; Araújo, Glaucy Rodrigues de; Miranda, Pedro Henrique de Amorim; Carneiro, Ana Cláudia Alvarenga; Ribeiro, Erica Milena de Castro; Silva, Breno de Mello; Lima, Wanderson Geraldo de; Costa, Daniela CaldeiraEthnopharmacological relevance: Baccharis trimera has been traditionally used in Brazil to treat liver diseases. Aim of the study: To evaluate the protective effect of Baccharis trimera in an ethanol induced hepatotoxicity model. Materials and methods: The antioxidant capacity was evaluated in vitro by the ability to scavenged the DPPH radical, by the quantification of ROS, NO and the transcription factor Nrf2. Hepatotoxicity was induced in animals by administration of absolute ethanol for 2 days (acute) or with ethanol diluted for 28 days (chronic). The biochemical parameters of hepatic function (ALT and AST), renal function (urea and creatinine) and lipid profile (total cholesterol, triglycerides and HDL) were evaluated. In addition to antioxidant defense (SOD, catalase, glutathione), oxidative damage markers (TBARS and carbonylated protein), MMP-2 activity and liver histology. Results: Baccharis trimera promoted a decrease in ROS and NO, and at low concentrations promoted increased transcription of Nrf2. In the acute experiment it promoted increase of HDL, in the activity of SOD and GPx, besides diminishing TBARS and microesteatosis. Already in the chronic experiment B. trimera improved the hepatic and renal profile, decreased triglycerides and MMP-2 activity, in addition to diminishing microesteatosis. Conclusion: We believe that B. trimera action is possibly more associated with direct neutralizing effects or inhibition of reactive species production pathways rather than the modulation of the antioxidant enzymes activity. Thus it is possible to infer that the biological effects triggered by adaptive responses are complex and multifactorial depending on the dose, the time and the compounds used.Item Baccharis trimera protege contra a hepatotoxicidade induzida pelo etanol em células HepG2 e em ratos.(2017) Rabelo, Ana Carolina Silveira; Costa, Daniela Caldeira; Lima, Wanderson Geraldo de; Saúde, Diena Antunes; Silva, André Talvani Pedrosa da; Costa, Daniela CaldeiraO etanol é o álcool mais utilizado nas bebidas alcóolicas e sua metabolização ocorre principalmente no fígado, sendo seu consumo abusivo associado à hepatotoxicidade. Um dos principais mecanismos responsáveis pela hepatotoxicidade induzida pelo etanol é o estresse oxidativo. Diante disso, os antioxidantes de origem exógena podem ser vistos como uma alternativa para restabelecer o equilíbrio oxidante/redutor, sendo esses amplamente encontrados nas plantas medicinais. Sendo assim, o objetivo desse trabalho foi verificar o efeito protetor dos extratos hidroetanólico e aquoso da espécie vegetal Baccharis trimera em modelo de hepatotoxicidade induzida com etanol. Para tal, foram realizados ensaios in vitro e in vivo, sendo utilizados os compostos quercetina e vitamina C, respectivamente, como controles positivos, já que o potencial antioxidante dos mesmos tem sido bem estabelecido. Os extratos de B. trimera foram avaliados quanto à citotoxicidade, sendo que apenas a concentração de 600 μg/mL do extrato hidroetanólico, no tempo de 24 horas, apresentou citotoxicidade. Foi observado que as células tratadas com os extratos de B. trimera e estimuladas com etanol apresentaram redução na produção de ERO e de óxido nítrico (NO), mas não apresentaram diferença significativa em relação ao fator de transcrição Nrf2. Os ensaios in vivo foram divididos em experimento agudo, onde ratos machos da linhagem Fisher foram pré-tratados com os extratos hidroetanólico e aquoso de B. trimera por dois dias; e experimento crônico, onde os animais foram pré-tratados apenas com o extrato hidroetanólico de B. trimera durante 28 dias. No experimento agudo foi observado que os extratos promoveram o aumento na concentração de glutationa oxidada e na atividade de glutationa peroxidase e superóxido dismutase. Também promoveram diminuição nos níveis de TBARS e melhora no perfil histológico, entretanto, não observamos melhora na função hepática e perfil lipídico destes animais. Já no experimento crônico, observamos melhora na função renal e hepática, diminuição nos níveis de triacilgliceróis, da atividade da metaloproteinase 2 e melhora no perfil histológico. Estes resultados analisados em conjunto sugerem que o extrato hidroetanolico de B. trimera melhora a hepatotoxicidade induzida pelo etanol tanto no modelo in vitro quanto in vivo. Palavras-chave: Etanol, hepatotoxicidade, estresse oxidativo, plantas medicinais,Item Estudos preliminares da citoxicidade e propriedades fotoprotetoras de derivados de Benzofenonas e Lactonas.(2018) Gonçalves, Marlucy da Cruz; Taylor, Jason Guy; Rossoni Júnior, Joamyr Victor; Rabelo, Ana Carolina Silveira; Costa, Daniela Caldeira; Cazati, Thiago; Santos, Viviane Martins Rebello dosA Radiação solar ultravioleta(RUV) pode induzir efeitos à pele devidos a sua ação direta ou indireta, por meio da geração de radicais livres. Esses efeitos podem provocar diversas lesões na pele humana como o câncer de pele. Como medida de proteção da pele contra os efeitos da radiação solar pode-se citar o uso de protetores solares, produtos tópicos adicionados de filtros solares UV sintéticos com propriedades de absorção e reflexão de raios solares.Um fotoprotetor orgânico ideal deve proteger a pele contra os raios UVB (290-320 nm) e UVA (320-400 nm), possuir um fator de proteção solar (FPS) seguro, ser fotoestável e não ser fototóxico. Este trabalho objetiva em estudos preliminares de fotoproteção dos derivados das Benzofenonas e Lactonas. Os produtos obtidos foram sintetizados e caracterizados por técnicas espectroscópicas usuais e foram submetidos a ensaios de viabilidade celular frente ao MTT e determinação do valor de proteção solar (FPS) in vitro pelo método espectrofotométrico UV/VIS. Os espectros de IV, RMN de 1 H e RMN de 13C mostraram bandas e sinais em conformidades com as estruturas propostas para os compostos estudados. Os compostos 1 e2 apresentaram um FPS proporcional à concentração analisada, ou seja, quanto maior a concentração, maior é o Fator de Proteção, porém o composto 3 apresentou fator proteção menor em concentrações mais elevadas. No estudo de viabilidade celular, os compostos 1 e 2 não foram citotóxicos nas concentrações avaliadas neste trabalho.Item Preparation and characterization of a quercetin-tetraethyl ether-based photoprotective nanoemulsion.(2019) Gonçalves, Marlucy da Cruz; Santos, Viviane Martins Rebello dos; Taylor, Jason Guy; Perasoli, Fernanda Barçante; Santos, Orlando David Henrique dos; Rabelo, Ana Carolina Silveira; Rossoni Junior, Joamyr Victor; Costa, Daniela Caldeira; Cazati, ThiagoAlthough Quercetin absorbs in the UVA/UVB electromagnetic region, it is limited for applications as a UV filter due to its low lipophilicity and capacity to penetrate the epidermis. In order to overcome this limitation, we synthetized and evaluated the photo protective properties of a derivative obtained from Quercetin. The derivative was prepared by alkylation of Quercetin with iodoethane and characterized by IR and NMR spectroscopy. The in vitro Solar Protection Factor was determined by the Mansur method and the cytotoxicity was evaluated using hepatocellular cell (Hep G2) cells. Finally, Quercetin and the corresponding derivative were incorporated in nanoemulsions. Nanoemulsions with particles sizes between 53 and 73 nm were obtained, and polydispersity indexes were around 0.1, indicating good homogeneity of the nanoemulsion particles. The cell viability study for the Quercetin derivative indicated a very low cytotoxicity profile. The chemical modification of Quercetin resulted in a promising compound with improved properties desirable for skin penetration and incorporation into sunscreen formulations.Item Recovering lead from cupel waste generated in gold analysis by Pb-Fire assay.(2016) Cerceau, Cristiane Isaac; Carvalho, Cornélio de Freitas; Rabelo, Ana Carolina Silveira; Varejão, Eduardo Vinícius VieiraBecause of its precision and accuracy, Pb-Fire assay is the most employed method for gold analysis in geological materials. At the second stage of the method, namely cupellation, lead is oxidized to PbO which is absorbed by the cupel, leading to metallic gold as a tiny bend at the bottom of the recipient. After cupellation, cupel becomes highly contaminated with lead, making its disposal a serious risk of environmental contamination. In the present work, a leaching process for removing lead from cupel waste is proposed, which allowed for removing 96% of PbO by weight. After a precipitation step, 92.0% of lead was recovered from leachates in the form of PbSO4. Lead in the solid wastes left by the extraction was above the limit established by Brazilian legislation and these were classified as non-hazardous. Finally, secondary effluents generated after the precipitation step presented lead content more than twenty times lower than that of leachates from cupel waste. Tons of cupel waste are annually generated from gold analysis by Pb-Fire assay. Thus, the proposed method can contribute to prevent the discharge of high amounts of lead into the environment. Also, recovery of lead can help to partially meet the industrial demand for lead compounds.Item A review of biological and pharmacological activities of Baccharis trimera.(2018) Rabelo, Ana Carolina Silveira; Costa, Daniela CaldeiraPlant-based systems continue to play an essential role in healthcare, and their use by different cultures has been extensively documented. Baccharis trimera, popularly known as carqueja, is a plant widely distributed in South America and has been traditionally used for treating several diseases particularly associated with hepatic and gastric disorders. The present study aimed to provide a general review of the available literature on phytochemical and biological data related to the species B. trimera as a potential source of new compounds with biological activity. Considering phytochemical studies, flavonoids, terpenes and chlorogenic acids were the main classes of compounds identified in aerial parts which were correlated with their biological activities such as antioxidant, anti-inflammatory, gastric and hepatic-protector, anti-microbial, anti-fungal, anti-parasitic and aid in weight loss.