Navegando por Autor "Pinto, Carla do Amaral"
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Item Caraparu virus induces damage and alterations in antioxidant defenses in the liver of BALB/c mice after subcutaneous infection.(2014) Camini, Fernanda Caetano; Almeida, Letícia Trindade; Bernardes, Carolina Silva; Silva, Maísa; Pedrosa, Maria Lúcia; Costa, Daniela Caldeira; Lima, Wanderson Geraldo de; Pinto, Carla do Amaral; Ferreira, Paulo César Peregrino; Magalhães, José Carlos de; Magalhães, Cíntia Lopes de BritoOxidative stress is a disturbance in the oxidantantioxidant balance leading to potential cellular damage. Most cells can tolerate a mild degree of oxidative stress because they have a system that counteracts oxidation that includes antioxidant molecules such as glutathione (GSH) and superoxide dismutase (SOD). Disruption of the host antioxidant status has been recognized as an important contributor to the pathogenesis of many viruses. Caraparu virus (CARV) is a member of group C of the Bunyaviridae family of viruses. In South American countries, group C bunyaviruses are among the common agents of human febrile illness and have caused multiple notable outbreaks of human disease in recent decades; nevertheless, little is known about the pathogenic characteristics of these viruses. The purpose of this study was to examine the hepatic pathogenesis of CARV in mice and the involvement of oxidative stress and antioxidant defenses on this pathology. Following subcutaneous infection of BALB/c mice, CARV was detected in the liver, and histopathology revealed acute hepatitis. Increased serum levels of aspartate and alanine aminotransferases (AST/ALT) and greater hepatic expression of the proinflammatory cytokine tumor necrosis factor- a (TNF-a) were found in infected animals. CARV infection did not alter the biomarkers of oxidative stress but caused an increase in GSH content and altered the expression and activity of SOD. This is the first report of an alteration of oxidative homeostasis upon CARV infection, which may, in part, explain the hepatic pathogenesis of this virus, as well as the pathogenesis of other Bunyaviridae members.Item Identification of a phylogenetically distinct orthobunyavirus from group C.(2011) Magalhães, Cíntia Lopes de Brito; Drumond, Betânia Paiva; Novaes, Renata Franco Vianna; Quinan, Bárbara Rezende; Magalhães, José Carlos de; Santos, João Rodrigues dos; Pinto, Carla do Amaral; Assis, Marieta Torres de Abreu; Bonjardim, Cláudio Antônio; Kroon, Erna Geessien; Ferreira, Paulo César PeregrinoApeu virus (APEUV) (family Bunyaviridae, genus Orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. Neutralising assays confirmed cross-reactivity between purified APEUV clones and the Caraparu virus complex of group C orthobunyaviruses. Partial sequencing of the L, M and S segments of one APEUV clone (APEUV-CL5) was carried out. A phylogenetic tree constructed with the L amino acid sequences clustered APEUV-CL5 within the genus Orthobunyavirus, confirming its serological classification. Analysis of M segment sequences clustered APEUV-CL5 in the Caraparu virus complex (Group C), in agreement with serological tests and previous molecular characterisation. However, the sequence of the nucleocapsid gene (N) gave low identity values when compared to those of the group C viruses. The phylogenetic tree based on N nucleotide sequences clustered APEUV-CL5 next to the California and Bwamba groups. This remarkable S nucleotide variability suggests that APEUV-CL5 could be a genetic reassortant and that this evolutionary mechanism is present in the history of the group C viruses.