Navegando por Autor "Paula, Carmem Aparecida de"
Agora exibindo 1 - 11 de 11
Resultados por página
Opções de Ordenação
Item Anti-inflammatory activity of Lychnophora passerina, Asteraceae (Brazilian Arnica).(2011) Oliveira, Patricia Capelari de; Paula, Carmem Aparecida de; Rezende, Simone Aparecida; Campos, Fernanda Torres; Guimarães, Andrea Grabe; Lombardi, Júlio Antônio; Guimarães, Dênia Antunes SaúdeEthnopharmacological relevance: Lychnophorapasserina (Asteraceae), popularly known as “arnica,” is used to treat inflammation, pain, rheumatism, contusions, bruises and insect bites in Brazilian traditional medicine. Materials and methods: The anti-inflammatoryactivity of crude ethanolic extract of aerial parts of L. passerina and its ethyl acetate and methanolic fractions had their abilities to modulate the production of NO, TNF-α and IL-10 inflammatory mediators in LPS/IFN-γ-stimulated J774.A1 macrophages evaluated. Moreover, the crude ethanolic extract and derived fractions were also in vivo assayed by carrageenan-induced paw oedema in mice. Results: In vitro assays showed remarkable anti-inflammatoryactivity of L. passerina crude ethanolic extract (EE) and its ethyl acetate (A) and methanolic (M) fractions, through the inhibition of production of NO and TNF-α inflammatory mediators and induction of production of IL-10 anti-inflammatory cytokine. In vivo assays showed anti-inflammatoryactivity for EE 10% ointment, similar to the standard drug diclofenac gel. The A and M fraction ointments 20% presented anti-inflammatoryactivity. Conclusion: The results obtained showed that possible anti-inflammatory effects of EE and its A and M fractions may be attributed to inhibition pro-inflammatory cytokines production, TNF-α and NO and to increased IL-10 production. EE, A and M ointments showed topical in vivoanti-inflammatoryactivity. The in vivoanti-inflammatoryactivity of EE of L. passerina may be related to synergistic effects of different substances in the crude extract. Therefore, traditional use of aerial parts of L. passerina in the inflammatory conditions could be beneficial to treat topical inflammatory conditions, as evidenced by the present study.Item Anti-inflammatory and antinociceptive activities of Campomanesia adamantium.(2013) Ferreira, Lidiane Cristina; Guimarães, Andrea Grabe; Paula, Carmem Aparecida de; Michel, Marcela Carolina de Paula; Guimarães, Raquel Gomes; Rezende, Simone Aparecida; Souza Filho, José Dias de; Guimarães, Dênia Antunes SaúdeEthnopharmacological relevance: Campomanesia species are used in folk medicine as anti-inflammatory, anti-rheumatic, anti-diarrheal and hypocholesterolemic. Aim of the study: The present study investigated the in vivo anti-inflammatory and antinociceptive properties of ethyl acetate (AE) and aqueous (Aq) extracts from leaves of Campomanesia adamantium and in vitro anti-inflammatory activity of AE and its isolated flavonols, myricitrin and myricetin. Materials and methods: The antinociceptive activity of AE and Aq was evaluated using acetic acid- induced writhing and formalin methods. The in vivo anti-inflammatory effect of AE and Aq was evaluated using carrageenan-induced paw oedema in mice. AE, myricitrin and myricetin were evaluated for their abilities to modulate the production of NO, TNF-a and IL-10 in LPS/IFN-g stimulated J774.A1 macrophages. Results: It was found that orally administrated AE and Aq (125 and 250 mg/kg) inhibited carrageenan- induced paw oedema in mice. AE (125 and 250 mg/kg) and Aq (125 mg/kg) reduced the time to licking at the second phase of the formalin method in vivo in mice. AE (250 mg/kg) and Aq (125 mg/kg) also reduced the number of writhes. AE, myricitrin and myricetin inhibited NO (320 mg/mL and 6.25–100 mM, respectively) and TNF-a production by macrophages (320 mg/mL for AE, 100 mM for myricitrin and 25–100 mM for myricetin). AE (160 and 320 mg/mL), myricitrin (50 and 100 mM) and myricetin (25–100 mM) increased IL-10 production by macrophages. Conclusions: The ethyl acetate and aqueous extracts from Campomanesia adamantium showed anti- nociceptive and anti-inflammatory effects supporting the use of the plant in folk medicine. The results suggest that anti-oedematogenic effect promoted by aqueous extract involves several anti- inflammatory mechanisms of action. The antinociceptive effect shown by aqueous extract can be due to the modulation of release of inflammatory mediators involved in nociception. The anti-inflammatory effects of AE and of its isolated flavonols may be attributed to inhibition of pro-inflammatory cytokines production, TNF-a and NO and to the increased of IL-10 production.Item Anti-inflammatory sesquiterpene lactones from Lychnophora trichocarpha Spreng. (Brazilian Arnica).(2013) Ferrari, Fernanda Cristina; Ferreira, Leidiane Cristina; Souza, Maíra Ribeiro de; Guimarães, Andrea Grabe; Paula, Carmem Aparecida de; Rezende, Simone Aparecida; Guimarães, Dênia Antunes SaúdeThe aerial parts of Lychnophora trichocarpha Spreng. (Asteraceae) are used macerated in water or ethanol to treat inflammation, pain, rheumatism, contusions, bruises and insect bites in Brazilian traditional medicine. In this study, anti-inflammatory activity of ethanol extract from aerial parts of L. trichocarpha and its ethyl acetate fraction was investigated. Sesquiterpene lactones, lychnopholide (Lyc) and eremantholide C (EreC), isolated of ethyl acetate fraction, were also assayed for in vitro and in vivo anti-inflammatory activity. Topical treatment with ointments containing ethanol extract, its ethyl acetate fraction and sesquiterpene lactones significantly reduced carrageenan-induced mice paw oedema. In vitro assays demonstrated that Lyc inhibited interferon - g/lipopolysaccharide -stimulated nitric oxide (NO) production in J774A.1 macrophages and increased production of IL-10 anti-inflammatory cytokine. The reduction of tumor necrosis factor-a (TNF-a) production by EreC was accompanied by an increased production of IL-10 in a concentration-dependent manner in J774A.1 macrophages. The anti-inflammatory effect of Lyc seems to involve the inhibition of production of NO and increased production of IL-10. The mechanism of the effect of EreC on the reduction of carrageenan-induced paw oedema may be attributed to inhibition of production of TNF-a and stimulation of IL-10 production. The results corroborate the use of ethanol extract from Lychnophora trichocarpha in folk medicine for anti-inflammatory action and indicate that the topical route is suitable for use.Item Avaliação da incidência de hemoglobinopatias em pacientes atendidos no laboratório piloto de análises clínicas da Escola de Farmácia – UFOP.(Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto., 2010) Nicolato, Roney Luiz de Carvalho; Carneiro, Cláudia Martins; Paula, Carmem Aparecida deAs hemoglobinopatias constituem um grupo de doenças de caráter genético caracterizadas pela síntese de cadeias polipeptídicas estruturalmente anormais ou diminuição da síntese de uma ou mais cadeias de globina. Dentre as variantes estruturais da hemoglobina, ou hemoglobinas anormais, as mais comuns são as hemoglobinas S e C, ambas resultantes da substituição de um único aminoácido na cadeia polipeptídica beta. Estudos realizados no Brasil mostram uma alta prevalência de heterozigotos para HbS e HbC. A frequência destas variantes na população brasileira é muito variável, pois está relacionada com os grupos raciais formadores de cada região. O povoamento da cidade de Ouro Preto, em 1698, motivado pela procura de ouro foi composto principalmente por portugueses e escravos africanos, contexto que favoreceu a mestiçagem entre os povos. Considerando que esses povos apresentam genes para as hemoglobinas anormais com frequências variadas, é esperado que se encontrem essas alterações genéticas na nossa população. O presente estudo teve como objetivo principal avaliar a frequência de hemoglobinas anormais nos pacientes atendidos pelo Laboratório Piloto de Análises Clínicas (LAPAC) da Escola de Farmácia da Universidade Federal de Ouro Preto (UFOP). No período de agosto de 2008 a setembro de 2010, foram triados 943 pacientes de ambos os sexos e diferentes faixas etárias para a frequência de variantes da hemoglobina, tendo como foco a hemoglobina falciforme, também conhecida como hemoglobina S. O diagnóstico foi realizado mediante a realização de eletroforese de hemoglobina em pH alcalino e as amostras positivas para alguma variante foram confirmadas por cromatografia líquida de baixa pressão (LPLC) e pelo teste de solubilidade. A prevalência de variantes estruturais de hemoglobina foi de 6,6%, sendo 4,88% para o traço falciforme (HbAS), 1,59 % para o genótipo AC e 0,11% para o genótipo SC. Não foram detectados indivíduos homozigotos para nenhum dos tipos de hemoglobinas variantes. Os resultados encontrados foram superiores aos encontrados nos estados de Pernambuco, Rio de Janeiro, Minas Gerais, São Paulo e Goiás, mostrando a dispersão dos genes para a HbS e HbC na população de Ouro Preto. Por essa razão, concluímos que é importante realizar programas com maior abrangência na população para estudo da epidemiologia das hemoglobinas variantes e de outras hemoglobinopatias resultantes de uma diminuição da síntese de uma ou mais cadeias de globina, no município de Ouro Preto.Item Effect of substrate and pH on the activity of proteases from Fusarium oxysporum var. lini.(1991) Castro, Ieso de Miranda; Lima, Angélica Alves; Paula, Carmem Aparecida de; Nicoli, Jacques Robert; Brandão, Rogélio LopesThe results obtained in this work suggest that both the pH (through selective inhibition) and the carbon source (through repression and acidification or alkalinization of the medium) may play an important role in the distribution of extracellular proteases in Fusarium oxysporum var. lini.Item Effects of extracts of leaves from Sparattosperma leucanthum on hyperuricemia and gouty arthritis.(2015) Lima, Rita de Cássia Lemos; Ferrari, Fernanda Cristina; Souza, Maíra Ribeiro de; Pereira, Bruna Maria de Sá; Paula, Carmem Aparecida de; Guimarães, Dênia Antunes SaúdeETHNOPHARMACOLOGICAL RELEVANCE: The species Sparattosperma leucanthum (Vell.) K. Schum is used in Brazilian folk medicine to treat rheumatism, throat ulcers, stomatitis, syphilis, bladder stones and as blood cleanser. The aim of this study was to evaluate the potential, in vitro and in vivo, of the extracts of leaves from Sparattosperma leucanthum to treat hyperuricemia and inflammation in the gouty arthritis model. MATERIALS AND METHODS: Ethyl acetate extract (SLE), methanolic extract (SLM) and aqueous extract (SLW) were evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity and liver xanthine oxidase (XOD) inhibition. Anti-inflammatory activity was also investigated on MSU crystal-induced paw edema model. RESULTS: Sparattosperma leucanthum crude extracts showed expressive results on urate-lowering activity in blood. SLW at the dose of 125 mg/kg has proved to be active in reducing hyperuricemia and was capable to inhibit the hepatic xanthine oxidase enzyme (XOD). SLM showed anti-hyperuricemic activity on all doses tested; however, this extract showed activity on the XOD only at the dose of 500 mg/kg. SLE, at the three evaluated doses, has proved to be active in reducing hyperuricemia in vivo and was able to inhibit XO activity in vitro at the concentration of 100 µg/mL. This extract was also able to inhibit XOD activity in vivo at the doses of 250 mg/kg and 500 mg/kg. SLE (125 and 250 mg/kg) and SLW (500 mg/kg) showed significant anti-inflammatory activity on monosodium urate crystal-induced paw edema model. CONCLUSIONS: The ethyl acetate, methanolic and aqueous extracts of Sparattosperma leucanthum showed significant results on evaluated models and therefore may be important agents for the treatment of gouty arthritis and hyperuricemia.Item Extracts from the leaves of Campomanesia velutina inhibits production of LPS/INF-γ induced inflammatory mediators in J774A.1 cells and exerts anti-inflammatory and antinociceptive effects in vivo.(2013) Michel, Marcela Carolina de Paula; Guimarães, Andrea Grabe; Paula, Carmem Aparecida de; Rezende, Simone Aparecida; Sobral, Marcos Eduardo Guerra; Guimarães, Dênia Antunes SaúdeCampomanesia velutina (Cambess) O. Berg, Myrtaceae, popularly known as “gabiroba” or “guavira”, is used in traditional Brazilian medicine to treat several diseases, including inflammation and rheumatism. Extraction and isolation from leaves of the plant afforded the active compound myricetin 3-O-rhamnoside, also known as myricitrin. The ethanolic extract of leaves of C. velutina and its ethyl acetate and methanolic fractions were evaluated in inflammation (carrageenan-induced paw oedema) and analgesic models (acetic acid-induced abdominal writhing and hot plate test). Moreover, the ethanolic extract, its fractions and the isolated compound were also in vitro evaluated for their ability to modulate NO, TNF-α and IL-10 production from J774A.1 macrophages stimulated by LPS/IFN-γ. In vivo assays showed remarkable anti-inflammatory activity of ethanolic extract, ethyl acetate and methanolic fractions. The antinociceptive activity of ethanolic extract and A was demonstrated in acetic acid-induced abdominal writhing test. In vitro assays demonstrated that ethyl acetate and methanolic fractions fraction and myricitrin inhibited NO production from macrophages J774A.1. Also Myricitrin induced production of IL-10 anti-inflammatory cytokine. None of the samples was able to inhibited TNF-α production. The results demonstrated for the first time the anti-inflammatory and antinociceptive activity of C. velutina.Item Hemocompatibilidade e efeito sobre o sistema imune de lipossomas vazios compostos de dicetilfosfato ou dipalmitoilfosfatidilglicerol.(2015) Costa, Walyson Coelho; Rezende, Simone Aparecida; Paula, Carmem Aparecida deLipossomas são sistemas de vetorização constituídos basicamente por fosfolipídios e colesterol e que têm sido amplamente usados como carreadores de fármacos, apresentando, dentre outras vantagens, a redução de efeitos adversos em comparação aos observados pelo uso de fármacos na forma livre. Devido ao fato possibilidade dos lipossomas serem naturalmente direcionados aos macrófagos, os quais são alvos da infecção por Leishmania, seu uso apresenta-se promissor para o tratamento das leishmanioses. Como ainda não existe disponível nenhuma formulação lipossomal contendo antimoniato de meglumina (AM), fármaco de primeira escolha na terapia das leishmanioses, a proposição de uma formulação contendo esse fármaco que apresente bons resultados no tratamento da doença e segurança adequada é alvo de inúmeras pesquisas. Formulações promissoras contendo AM têm sido propostas, porém algumas reações adversas têm sido apontadas pelo uso desse sistema, as quais parecem estar relacionadas ao uso do fosfolipídio dicetilfosfato (DCP). Assim, foi proposta uma nova formulação lipossomal em que o DCP foi substituído pelo fosfolipídio dipalmitoilfosfatidilgicerol (DPPG) com o objetivo de tentar reduzir as reações adversas observadas. Dessa forma, neste trabalho, foram realizados testes in vitro para avaliar o efeito das duas formulações vazias sobre componentes celulares e plasmáticos, a saber, a avaliação do efeito sobre as hemácias e sobre a hemostasia. Também foram realizados testes para avaliação do efeito das vesículas lipossomais sobre a linhagem J774 de macrófagos, através da determinação da viabilidade celular e da produção de óxido nítrico, e o efeito sobre o sistema complemento, avaliado pela formação de C3a e de SC5b-9. Os resultados encontrados no presente estudo mostraram que as duas formulações não tiveram efeito sobre a maioria dos testes realizados. Porém, a formulação lipossomal constituída por DCP provocou ativação do sistema complemento, com aumento da formação de C3a. Além disso, essa formulação provocou uma diminuição da viabilidade de macrófagos. Esses dados sugerem que as reações adversas observadas em estudos anteriores podem ser devido ao DCP presente na vesícula lipossomal.Item Kinetic characterization and inhibition of the rat MAB elastase-2, an angiotensin I-converting serine protease.(2002) Santos, Carlos F.; Paula, Carmem Aparecida de; Salgado, Maria Cristina de Oliveira; Oliveira, Eduardo Brandt deAn elastase-2 has been recently described as the major angiotensin (Ang) II-forming enzyme of the rat mesenteric arterial bed (MAB) perfusate. Here, we have investigated the interaction of affinity-purified rat MAB elastase-2 with some substrates and inhibitors of both pancreatic elastases-2 and Ang II-forming chymases. The Ang II precursor [Pro11-D-Ala12]-Ang I was converted into Ang II by the rat MAB elastase-2 with a catalytic efficiency of 8.6 min–1·mM–1, and the chromogenic substrates N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide and N-succinyl-Ala-Ala-Pro- Phe-p-nitroanilide were hydrolyzed by the enzyme with catalytic efficiencies of 10.6 min–1·mM–1 and 7.6 min–1·mM–1, respectively. The non-cleavable peptide inhibitor CH-5450 inhibited the rat MAB elastase-2 activities toward the substrates Ang I (IC50 = 49 mM) and N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (IC50 = 4.8 mM), whereas N-acetyl-Ala- Ala-Pro-Leu-chloromethylketone, an effective active site-directed inhibitor of pancreatic elastase-2, efficiently blocked the Ang II-generating activity of the rat MAB enzyme (IC50 = 4.5 mM). Altogether, the data presented here confirm and extend the enzymological similarities between pancreatic elastase-2 and its rat MAB counterpart. Moreover, the thus far unrealized interaction of elastase-2 with [Pro11-D-Ala12]-Ang I and CH-5450, both regarded as selective for chymases, suggests that evidence for the in vivo formation of Ang II by chymases may have been overestimated in previous investigations of Ang II-forming pathways.Item Pharmacological basis for use of Lychnophora trichocarpha in gouty arthritis : anti-hyperuricemic and anti-inflammatory effects of its extract, fraction and constituents.(2012) Souza, Maíra Ribeiro de; Paula, Carmem Aparecida de; Resende, Michelle Luciane Pereira de; Guimarães, Andrea Grabe; Souza Filho, José Dias de; Guimarães, Dênia Antunes SaúdeEthnopharmacological relevance: The ethanolic extract of Lychnophoratrichocarpha Spreng. is used in Brazilian folk medicine to treat bruise, pain and inflammatory diseases. Aim of the study: The present study aimed at investigating whether ethanolic extract of L. trichocarpha, its ethyl acetate fraction and its main bioactive compounds could be useful to treat gouty arthritis by countering hyperuricemia and inflammation. Materials and methods: L. trichocarpha ethanolic extract (LTE), ethyl acetate fraction from ethanolic extract (LTA) and isolated compounds were evaluated for urate-lowering activity and liver xanthine oxidase (XOD) inhibition in oxonate-induced hyperuricemic mice. Anti-inflammatory activity in monosodium urate crystal-induced paw oedema, an experimental model of gouty arthritis, was also investigated. Results: Crude ethanolic extract and its ethyl acetate fraction showed significant urate-lowering effects. LTE was also able to significantly inhibit liver xantine oxidase (XOD) activity in vivo at the dose of 250 mg/kg. Luteolin, apigenin, lupeol, lychnopholide and eremantholide C showed the anti-hyperuricemic activities among tested compounds. Apigenin also showed XOD inhibitory activity in vivo. Luteolin, lychnopholide, lupeol and eremantholide C, in turn, did not shown significant inhibitory activity towards this enzyme, indicating that this mechanism is not likely to be involved in urate-lowering effects of those compounds. LTE, LTA, lupeol, β-sitosterol, lychnopholide, eremantholide, luteolin and apigenin were also found to inhibit monosodium urate crystals-induced paw oedema in mice. Conclusions: Ethanolic extract of Lychnophoratrichocarpha and some of its bioactive compounds may be promising agents for the treatment of gouty arthritis since they possesses both anti-hiperuricemic and anti-inflammatory properties.Item Purification and substrate specificity of an angiotensin converting elastase-2 from the rat mesenteric arterial bed perfusate.(1998) Paula, Carmem Aparecida de; Sousa, Marcelo Valle de; Salgado, Maria Cristina de Oliveira; Oliveira, Eduardo Brandt deA soluble angiotensin (Ang) II-generating enzyme has been purified to homogeneity from the rat mesenteric arterial bed(MAB) perfusate by a combination of gel filtration and affinity chromatographies. The enzyme is a glycoprotein of 28.5 kDa(SDS-PAGE), whose N-terminal sequence is identical with that of the rat pancreatic elastase-2 ; therefore the enzyme willhenceforth be referred to as rat MAB elastase-2. When Ang I was used as the substrate, the enzyme specifically released AngII and the dipeptide His-Leu (Km=36WM;Kcat= 1530 min31). The catalytic efficiency (Kcat/Km= 42.5 min31WM31) of thisreaction was comparable to those of other known Ang I-converting enzymes. The proteolytic specificity of the purifiedenzyme toward mellitin, oxidized insulin B chain, somatostatin-14 and renin substrate tetradecapeptide suggested that theenzyme-substrate interaction was defined by an extended substrate binding site, typical of elastases-2 of pancreatic origin. According to the sensitivity of the rat MAB elastase-2 to various inhibitors this enzyme could be described as a member ofthe chymostatin-sensitive group of Ang II-forming serine proteases. The localization and biochemical properties of thisenzyme suggest that it might play a role in the regional control of vascular tonus.