Navegando por Autor "Machado, Kamilla Nunes"
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Item Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells : in vitro and in vivo traditional and replacement methods.(2022) Ferreira, Paulo Michel Pinheiro; Sousa, Ian Jhemes Oliveira; Machado, Kamilla Nunes; Silva Neto, Leonel Antônio da; Freitas, Milena Monteiro de; Santos, Ingredy Lopes dos; Rodrigues, Débora Caroline do Nascimento; Sousa, Rayran Walter Ramos de; Reis, Antonielly Campinho dos; Nascimento, Maria Luisa Lima Barreto do; Menezes, Ag-Anne Pereira Melo de; Nascimento, Andréa Mendes do; Ferreira, José Roberto de Oliveira; Peron, Ana Paula; Sousa, João Marcelo de Castro eStevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina, erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal damage. The assessment using A. salina larvae and mouse blood cells revealed LC50 and EC50 values of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and chromosomal aberrations were detected only at 100 μg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing ABTS• and DPPH• . No previous in vivo studies were performed before with the EtAcSur. Signals of acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25 and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity were not observed, suggesting the significant pharmacological potential of S. urticifolia for the development of antineoplastic agents.Item Biological screening of extracts of Brazilian Asteraceae plants.(2013) Carvalho, Cintia Cristina de; Machado, Kamilla Nunes; Ferreira, Paulo Michel Pinheiro; Pessoa, Cláudia; Fonseca, Thaisa Helena Silva; Gomes, Maria Aparecida; Nascimento, Andréa Mendes doNatural products are a very productive source of leads for the development of medicines. Seven Brazilian Asteraceae adult plants were randomly chosen. The current study was designed to evaluate the antiprotozoal and cytotoxic activities in vitro of 21 extracts obtained. Phytochemical properties of the most active extracts also were checked. Cytotoxic activity was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines (HCT-116, OVCAR 8 and SF-295). The antiprotozoal activity was evaluated against Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. None of the extracts showed antiprotozoal activity. However, 76% of the extracts displayed moderate to high in vitro cytotoxic activities against human cancer cell which suggests that they are a promising source of anticancer compound, since none of the extracts showed hemolytic activity. Terpenoids, flavonoids, saponins, tannins, besides other compound classes, were identified and may be responsible for their antitumor activity. Cytotoxic assays indicate the anticancer potential of Asteraceae species from Brazil.Item Flavonoids, antioxidant, and antiproliferative activities of Stevia urticifolia.(2017) Machado, Kamilla Nunes; Hernández Tasco, Alvaro José; Salvador, Marcos José; Rodrigues, Ivanildes Vasconcelos; Pessoa, Claudia do Ó; Sousa, Ian Jhemes Oliveira; Ferreira, Paulo Michel Pinheiro; Nascimento, Andréa Mendes do