Navegando por Autor "Ferreira, Paulo Michel Pinheiro"
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Item Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells : in vitro and in vivo traditional and replacement methods.(2022) Ferreira, Paulo Michel Pinheiro; Sousa, Ian Jhemes Oliveira; Machado, Kamilla Nunes; Silva Neto, Leonel Antônio da; Freitas, Milena Monteiro de; Santos, Ingredy Lopes dos; Rodrigues, Débora Caroline do Nascimento; Sousa, Rayran Walter Ramos de; Reis, Antonielly Campinho dos; Nascimento, Maria Luisa Lima Barreto do; Menezes, Ag-Anne Pereira Melo de; Nascimento, Andréa Mendes do; Ferreira, José Roberto de Oliveira; Peron, Ana Paula; Sousa, João Marcelo de Castro eStevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina, erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal damage. The assessment using A. salina larvae and mouse blood cells revealed LC50 and EC50 values of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and chromosomal aberrations were detected only at 100 μg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing ABTS• and DPPH• . No previous in vivo studies were performed before with the EtAcSur. Signals of acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25 and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity were not observed, suggesting the significant pharmacological potential of S. urticifolia for the development of antineoplastic agents.Item Antiproliferative and photoprotective activities of the extracts and compounds from Calea fruticosa.(2020) Seregheti, Thatiane M. Q.; Pinto, Ana Paula Resende Seibert; Gonçalves, Marlucy da Cru; Antunes, Amanda dos Santos; Almeida, Wanessa Aparecida da Silveira; Machado, Ranyelison Silva; Silva, Jurandy N.; Ferreira, Paulo Michel Pinheiro; Pessoa, Claudia; Santos, Viviane Martins Rebello dos; Nascimento, Andréa Mendes doIn this paper, we complement our previous study on the antiproliferative activity of Calea fruticosa (Asteraceae) by isolating the compounds apigenin-4’,7-dimethyl ether (1), budlein A (2), quercetin (3), and cichoriin (4) from the plant’s aerial parts. The antiproliferative activity of these compounds was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines. Compound 3 displayed moderate antiproliferative activity in three cell lines (HCT 116, PC-3, and SF-295, with cell growth inhibition values of 72.97, 74.55, and 68.94%) and high antiproliferative activity (90.86%) in the HL-60 cell line. The in vitro sun protection factor (SPF) of the extracts and compound 4, with and without sunscreen, was determined by a spectrophotometric method. The ethanol extract exhibited the highest SPF (9.67) at a concentration of 0.100 mg/mL, while compound 4, isolated from this extract, showed a SPF of 13.79 at the same concentration. A relative increased efficacy of SPF was observed for the extracts and compound 4 when sunscreen was also used. Compound 4 has not been reported previously from any species within the genus Calea. Compounds 1–4 were obtained from this species for the first time.Item Biological screening of extracts of Brazilian Asteraceae plants.(2013) Carvalho, Cintia Cristina de; Machado, Kamilla Nunes; Ferreira, Paulo Michel Pinheiro; Pessoa, Cláudia; Fonseca, Thaisa Helena Silva; Gomes, Maria Aparecida; Nascimento, Andréa Mendes doNatural products are a very productive source of leads for the development of medicines. Seven Brazilian Asteraceae adult plants were randomly chosen. The current study was designed to evaluate the antiprotozoal and cytotoxic activities in vitro of 21 extracts obtained. Phytochemical properties of the most active extracts also were checked. Cytotoxic activity was evaluated by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines (HCT-116, OVCAR 8 and SF-295). The antiprotozoal activity was evaluated against Trichomonas vaginalis, Entamoeba histolytica and Giardia lamblia. None of the extracts showed antiprotozoal activity. However, 76% of the extracts displayed moderate to high in vitro cytotoxic activities against human cancer cell which suggests that they are a promising source of anticancer compound, since none of the extracts showed hemolytic activity. Terpenoids, flavonoids, saponins, tannins, besides other compound classes, were identified and may be responsible for their antitumor activity. Cytotoxic assays indicate the anticancer potential of Asteraceae species from Brazil.Item Flavonoids, antioxidant, and antiproliferative activities of Stevia urticifolia.(2017) Machado, Kamilla Nunes; Hernández Tasco, Alvaro José; Salvador, Marcos José; Rodrigues, Ivanildes Vasconcelos; Pessoa, Claudia do Ó; Sousa, Ian Jhemes Oliveira; Ferreira, Paulo Michel Pinheiro; Nascimento, Andréa Mendes doItem In vitro activity evaluation of seven Brazilian Asteraceae against cancer cells and Leishmania amazonensis.(2019) Silva, Ana Cláudia Nogueira da; Nascimento, Renato Malveira Carreiro do; Rodrigues, Débora Caroline do Nascimento; Ferreira, Paulo Michel Pinheiro; Pessoa, Claudia do Ó; Lima, Daisy Jereissati Barbosa; Moraes Filho, Manoel Odorico de; Almeida, Raquel Martins de; Ferreira, Sebastião Rodrigo; Fujiwara, Ricardo Toshio; Nascimento, Andréa Mendes doNatural products are a very productive leading source of compounds for the development of medicines. The aim of this research was to investigate the in vitro antiproliferative and antileishmanial activities of 21 extracts from 7 Asteraceae species. Dried aerial parts of Asteraceae plant species were extracted using organic solvents in order of increasing polarities. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and nine extracts (43%) displayed moderate to high in vitro antiproliferative activity against human cancer cell lines HCT-116, OVCAR-8 and SF-295. Two crude extracts displaying antiproliferative activity were fractionated and yielded a fraction rich in lupeol acetate, a fraction rich in lupeol and 5,7,3′,4′-tetrahydroxyflavone-7-O-β-D-glucopyranoside. The antileishmanial activity was evaluated by amastigotes growth inhibition test in Leishmania amazonensis, and three extracts showed promising activity. Phytochemical screening of all plant extracts was also made for terpenoids, flavonoids, tannins, alkaloids and saponins.