Navegando por Autor "Ferreira, Fernanda Lopes"
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Item Evaluation of the activity of Tontelea micrantha extracts against Bacteria, Candida and Mayaro virus.(2018) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Lima, William Gustavo de; Moraes, Thaís de Fátima Silva; Ferreira, Fernanda Lopes; Ferreira, Jaqueline Maria Siqueira; Magalhães, Cíntia Lopes de Brito; Duarte, Lucienir Pains; Vieira Filho, Sidney Augusto; Magalhães, José Carlos deObjectives: This work aimed to evaluate the antibacterial, antifungal, and anti-Mayaro virus(MAYV) activity of leaf and branch extracts from Tontelea micrantha. Materials and Methods: T. micrantha extracts were prepared through the partition of the leaf and branch samples in different solvents. Then, the antibacterial and antifungal activity was assessed against bacterial pathogens and Candida sp. by the determination of the minimum inhibitory concentration (MIC) by the broth microdilution method. The activity against anti-MAYV was evaluated through the quantification of the extract concentration that promoted the protection of 50% of the cells after the viral infection. Results: The extracts of T. micrantha were inactive (MIC >500 µg/mL) against Gram-positive, Gram-negative and Candida species at the highest concentration tested (500 µg/mL). Anti-MAYV activity was also not detected, with SI <10, ranging from 1.2 to 3.6. Conclusion: Although it is used in traditional medicine, Leaf and branch extracts from T. micrantha did not present antimicrobial activity, which could be caused by the antagonistic effect of the compounds present in the extract.Item Lack of activity of rutin isolated from Tontelea Micrantha leaves against Vero and BHK, Fungi, Bacteria and Mayaro virus and its in silico activity.(2020) Nizer, Waleska Stephanie da Cruz; Ferraz, Ariane Coelho; Moraes, Thaís de Fátima Silva; Ferreira, Fernanda Lopes; Magalhães, Cíntia Lopes de Brito; Vieira Filho, Sidney Augusto; Duarte, Lucienir Pains; Magalhães, José Carlos deThis work aimed to evaluate the antibacterial, antifungal, and anti-Mayaro virus (MAYV) activity of rutin, a flavonoid isolated from the leaves of Tontelea micrantha (Celastraceae). Materials and Methods: The antibacterial and antifungal activities were evaluated by the broth microdilution method through the determination of the minimum inhibitory concentration. The anti-MAYV activity was determined by the rutin concentration required to protect 50% of the cells after viral infection. The indirect 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay was used to determine the cytotoxic concentration of rutin to 50% of Vero and BHK cells. The antimicrobial activity spectrum of rutin was predicted using the online software prediction of activity spectra for substances (PASS). Results: Although the PASS prediction had shown that a higher probability of rutin acts as an antifungal agent, the in vitro assays showed no antimicrobial activity. Regarding the cytotoxicity assay, rutin was not toxic to Vero and BHK cells. Conclusions: Many biological activities have been described for flavonoids, but the flavone rutin did not show antimicrobial activity in vitro. The results obtained in this work suggest that rutin is not a promising antimicrobial agent.