Navegando por Autor "Estevam, Charles dos Santos"

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    Analysis of oxidative stress in Wistar rats submitted to high‐intensity interval training.
    (2020) Souza, Lúcio Marques Vieira; Martins, Felipe José Aidar; Matos, Dihogo Gama de; Marçal, Anderson Carlos; Souza, Raphael Fabrício de; Santos, Jymmys Lopes dos; Wartha, Elma Regina Silva de Andrade; Silva, Albená Nunes da; Estevam, Charles dos Santos; Araújo, Silvan Silva de
    Physical exercise is a known factor that can promote oxidative stress and may result in cellular damage if not neutralized by antioxidant mechanisms. This study aimed to determine if the level of hepatic oxidative stress resulting from high-intensity interval training (HIIT) is affected by the frequency pattern (consecutive vs. non-consecutive) of the training sessions. Thirty-two Wistar rats were divided equally into four groups: two control groups, (CS1) and (CS2), remained sedentary throughout the experiment, and two test groups, (CT1) and (CT2), and were submitted to HIIT for 12 consecutive and non-consecutive (12 sessions, 3 times/wk over four weeks) days, respectively. There were no significant differences in markers of oxidative damage measured in hepatic tissue (TBARS) and markers of antioxidant activity (Sulfhydryl, FRAP), as well as markers of hepatic damage (AST and ALT) and antioxidant defense (Uric Acid) measured in plasma of both HIIT groups after training compared to the control groups. The results indicate that both HIIT performed for 12 consecutive and non-consecutive sessions did not promote hepatic oxidative damage in rats.
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    Antinociceptive and anti-inflammatory effect of Poincianella pyramidalis (Tul.) L.P. Queiroz.
    (2020) Moraes, Sabrina Zelice da Cruz de; Shan, Andrea Yu Kwan Villar; Melo, Marlange Almeida Oliveira; Silva, Juliane Pereira da; Passos, Fabiolla Rocha Santos; Graça, Ariel de Souza; Araújo, Brancilene Santos de; Quintans, Jullyana de Souza Siqueira; Quintans Júnior, Lucindo José; Barreto, Emiliano de Oliveira; Brandão, Geraldo Célio; Estevam, Charles dos Santos
    Ethnopharmacology relevance: Poncianella pyramidalis (Leguminosae) is a Caatinga plant used in folk medicine because of its pharmacological properties, which include anti-inflammatory action. However, chemical compounds re sponsible for this effect have not yet been identified. Aim of the study: This study aimed to evaluate the antioxidant, antinociceptive and anti-inflammatory effects of the ethyl acetate fraction from the inner bark of P. pyramidalis. Material and methods: Total phenol content (TP) was estimated using the Folin-Ciocalteu reagent, while in vitro antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Chemical identification was done using LC-PDA/MS and LC-ESI/MS/MS. In vivo antinociceptive and anti-in flammatory properties were investigated using formalin, mechanical hypernociception and carrageenan-induced pleurisy assays in mice. Results: TP was 525.08 ± 17.49 μg mg−1 gallic acid equivalent. The ethyl acetate fraction (EAF) inhibited 87.76% of the DPPH radical with an EC50 of 22.94 μg mL−1 and Antioxidant Activity Index of 1.74. LC-PDA/MS and LC-ESI/MS/MS identified 15 compounds that are mostly derived from gallic and ellagic acids. Regarding in vivo antinociceptive and anti-inflammatory activity, EAF (100 mg kg-1) significantly reduced the nociceptive response in the second phase of the formalin assay by 50% (p < 0.01) compared with the control group. In the hypernociception test, a significant (p < 0.001) anti-hyperalgesic effect of EAF (100 mg kg-1) was observed up to the third hour of evaluation (p < 0.001). In the carrageenan assay, EAF (100 mg kg-1) was shown to inhibit protein extravasation, increase total leukocytes and neutrophils, and inhibit mononuclear cells. Conclusion: These results demonstrate EAF from the inner bark of P. pyramidalis has strong in vitro antioxidant effect as well as in vivo antinociceptive and anti-inflammatory activities, which may be attributed to the bark being rich in phenolic compounds derived from gallic acid.
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    Does Croton argyrophyllus extract has an effect on muscle damage and lipid peroxidation in rats submitted to high intensity strength exercise?
    (2019) Araújo, Silvan Silva de; Martins, Felipe José Aidar; Matos, Dihogo Gama de; Santos, Jymmys Lopes dos; Souza, Lúcio Marques Vieira; Silva, Albená Nunes da; Santos, Rodrigo Miguel dos; Marçal, Anderson Carlos; Mourão, Daniella Mota; Júnior, Amário Lessa; Durães, Geraldo Magela; Carneiro, André Luiz Gomes; Silva, Rodrigo Gonçalves da; Teixeira, Mauro Martins; Estevam, Charles dos Santos
    Many species of the genus Croton have been used for anti-inflammatory, antiproliferative, antidiabetic, and antitumor purposes. The objective was to evaluate the e ect of a hydroethanolic extract (HEE) from the inner bark of Croton argyrophyllus (Euphorbiaceae) on muscle damage and oxidative stress in rats after high intensity exercise. The animals were divided into four groups: (i) the sedentary group (SV; n = 7), (ii) the exercise vehicle group (EV, n = 7), (iii) the sedentary group HEE (SHG; n = 7) composed of sedentary animals and treated with the hydroethanolic extract of C. argyrophyllus (200 mg/kg, v.o.), and (iv) the HEE exercise group (HEE; n = 7) composed of animals submitted to resistance exercise (RE) and treated with the hydroethanolic extract of C. argyrophyllus (200 mg/kg, v.o.). In the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) test, the HEE showed lower values of inhibition potential (IP%) at 39.79% compared to gallic acid, 87.61%, and lipoperoxidation inhibition at 27.4% (100 µg/mL) or 28.6% (200 µg/mL) (p < 0.001). There was inhibition in free radicals in vivo. The HEE of C. argyrophyllus partially reduced the biomarkers of oxidative stress in muscle tissue and muscular damage (creatine kinase (CK) and Lactate Dehydrogenase (LDH)) (p < 0.05) in rats, and in this sense it can be an aid to the recovery process after exhaustive e orts.
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    Effects of resistance training and Bowdichia virgilioides hydroethanolic extract on oxidative stress markers in rats submitted to peripheral nerve injury.
    (2020) Costa, Luana Santos; Martins, Felipe José Aidar; Matos, Dihogo Gama de; Oliveira, José Uilien de; Santos, Jymmys Lopes dos; Almeida Neto, Paulo Francisco de; Souza, Raphael Fabrício de; Pereira, Danielle Dutra; Garrido, Nuno Domingos; Silva, Albená Nunes da; Marçal, Anderson Carlos; Estevam, Charles dos Santos; Cabral, Breno Guilherme de Araújo Tinôco; Reis, Victor Machado; Teixeira, Mauro Martins
    The objective of this study was to analyze the effects of the combination of resistance training (RT) and the hydroethanolic extract (EHE) of Bowdichia virgilioides as markers of oxidative stress (OS) in rats with peripheral nerve injury (PNI). Rats were allocated into six groups (n = 10): animals without interventions (C), animals with an exposed nerve but without injury, injured animals, trained and injured animals, injured animals that received EHE, and animals that received a combination of RT and EHE. RT comprised the climbing of stairs. EHE was orally administered (200 mg/kg) for 21 days after PNI induction. RT reduced the amount of lipoperoxidation in plasma (14.11%). EHE reduced lipoperoxidation in the plasma (20.72%) and the brain (41.36). RT associated with the extract simultaneously reduced lipoperoxidation in the plasma (34.23%), muscle (25.13%), and brain (43.98%). There was an increase in total sulhydrilyl levels (a) in the brain (33.33%) via RT; (b) in the brain (44.44%) and muscle (44.51%) using EHE; and (c) in the plasma (54.02%), brain (54.25%), and muscle using the combination of RT + EHE. These results suggest that RT associated with oral EHE results in a decrease in OS.
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    HIIT de curto prazo não promove estresse oxidativo ou danos musculares.
    (2021) Souza, Lúcio Marques Vieira; Martins, Felipe José Aidar; Matos, Dihogo Gama de; Silva, Albená Nunes da; Santos, Rodrigo Miguel dos; Santos, Jymmys Lopes dos; Costa, Rôas de Araújo; Marçal, Anderson Carlos; Santos, Sandra Lauton; Cabral, Breno Guilherme de Araújo Tinôco; Estevam, Charles dos Santos; Araújo, Silvan Silva de
    Introdução: O treinamento intervalado de alta intensidade (HIIT) é um método muito utilizado atualmente. Objetivo: O presente estudo objetivou avaliar os efeitos do HIIT em curto prazo sobre marcadores de estresse oxidativo e dano muscular em ratos. Métodos: A amostra consistiu em ratos Wistar com 60 dias de idade, divididos em dois grupos: grupo controle (n = 8) e grupo HIIT (n = 8). O treinamento consistiu em quatorze sessões de natação de 20 segundos (com cargas equivalentes a 14% do peso corporal) com intervalos de 10 segundos entre cada sessão, realizadas por 12 dias consecutivos. Resultados: O HIIT induziu uma redução (-17,75%) das substâncias reativas ao ácido tiobarbitúrico (um marcador de estresse oxidativo) no tecido hepático (p = 0,0482). Houve também redução (-31,80%) no grupo HIIT no nível de enzima superóxido dismutase no fígado (p = 0,0375). No entanto, não houve diferenças entre os grupos com relação a catalase, glutationa peroxidase, glutationa redutase, teor total de sulfidrilas SH, hidroperóxidos ou proteínas carboniladas no tecido hepático. Nenhuma diferença significativa foi encontrada em qualquer um desses mascadores no músculo gastrocnêmio. Os marcadores de lesão muscular, creatinina quinase e lactato desidrogenase, também foram semelhantes entre os grupos no gastrocnêmio. Conclusão: Foi possível concluir que o HIIT de curta duração não causa estresse oxidativo ou dano muscular. Nível de evidência I; Estudo clínico randomizado de alta qualidade com ou sem diferença estatisticamente significante, mas com intervalos de informação estreitos.
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    The positive inotropic effect of the ethyl acetate fraction from Erythrina velutina leaves on the mammalian myocardium : the role of adrenergic receptors.
    (2013) Passos, Amilton Gustavo da Silva; Gondim, Antônio Nei Santana; Campos, Danilo Roman; Cruz, Jader dos Santos; Garcia, Eduardo Antônio Conde; Araújo Neto, Vitor; Estevam, Charles dos Santos; Cerqueira, Sandra Valéria Santos; Brandão, Geraldo Célio; Oliveira, Alaíde Braga de; Vasconcelos, Carla Maria Lins de
    Objectives We studied the effects of ethyl acetate fraction (EAcF) obtained from Erythrina velutina leaves on mammalian myocardium. Methods The effect of EAcF on the contractility was studied using guinea-pig left atria mounted in a tissue bath (Tyrode’s solution, 29°C, 95% CO2, 5% O2) and electrically stimulated (1 Hz). Concentration-response curves of EAcF were obtained in the presence of propranolol (1 mm), nifedipine (1 mm) and in reserpinized animals (5 mg/kg). The involvement of l-type calcium current (ICa,L) on the EAcF effect was observed in cardiomyocytes of mice assessed using patch-clamp technique. Key findings EAcF (550 mg/ml) had a positive inotropic effect, increasing the atrial force by 164% (EC50 = 157 44 mg/ml, n = 6), but it was less potent than isoproterenol (EC50 = 0.0036 0.0019 mg/ml, n = 8). The response evoked by EAcF was abolished by propranolol or nifedipine. Reserpine did not alter the inotropic response of EAcF. Furthermore, an enhancement of the ICa,L peak (31.2%) with EAcF was observed. Chemical analysis of EAcF revealed the presence of at least 10 different flavonoid glycoside derivatives. Two were identified as vicenin II and isorhoifolin. Conclusions We conclude that EAcF increases the cardiac contractile force by increasing the l-type calcium current and activating the adrenergic receptor pathway.