Navegando por Autor "Carneiro, Ana Paula Scalia"
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Item Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?(2020) Sauvain, Jean-Jacques; Edmé, Jean Louis; Wild, Pascal; Suárez, Gina Wirz; Bezerra, Olívia Maria de Paula Alves; Silva, André Talvani Pedrosa da; Algranti, Eduardo; Carneiro, Ana Paula Scalia; Chérot-Kornobis, Nathalie; Sobaszek, Annie; Hulo, SébastienExposure to environmental and occupational particulate matter(PM)induces health effects on the cardio-pulmonary system. In addition, associations between exposure to PM and metabolic syndromes like diabetes mellitus or obesity are now emerging in the literature. Collection of exhaled breath condensate (EBC) is an appealing non-invasive technique to sample pulmonary fluids. This hypothesis-generating study aims to (1) validate an ion chromatography method allowing the robust determination of different metabolism-related molecules(lactate, formate, acetate, propionate, butyrate, pyruvate, nitrite, nitrate)in EBC; (2) apply this method to EBC samples collected from workers exposed to quartz (a known inflammatory particle), to soapstone (a less inflammatory particle than quartz), as well as to controls. A multi-compound standard solution was used to determine the linearity range, detection limit, repeatability and bias from spiked EBC. The biological samples were injected without further treatment into an ion chromatograph with a conductivity detector. RTube® were used for field collection of EBC from 11 controls, 55 workers exposed to soapstone and 12 volunteers exposed to quartz dust. The analytical method used proved to be adequate for quantifying eight anions in EBC samples. Its sub-micromolar detection limits and repeatability, combined with a very simple sample preparation, allowed an easy and fast quantification of different glycolysis or nitrosative stress metabolites. Using multivariate discriminant analysis to maximize differences between groups, we observed a different pattern of anions with a higher formate/acetate ratio in the EBC samples for quartz exposed workers compared to the two other groups. We hypothesize that a modification of the metabolic signature could be induced by exposure to inflammatory particles like quartz and might be observed in the EBC via a change in the formate/acetate ratio.Item Does exposure to inflammatory particles modify the pattern of anion in exhaled breath condensate?(2020) Sauvain, Jean-Jacques; Edmé, Jean Louis; Wild, Pascal; Suárez, Gina Wirz; Bezerra, Olívia Maria de Paula Alves; Silva, André Talvani Pedrosa da; Algranti, Eduardo; Carneiro, Ana Paula Scalia; Chérot-Kornobis, Nathalie; Sobaszek, Annie; Hulo, SébastienExposure to environmental and occupational particulate matter(PM)induces health effects on the cardio-pulmonary system. In addition, associations between exposure to PM and metabolic syndromes like diabetes mellitus or obesity are now emerging in the literature. Collection of exhaled breath condensate (EBC) is an appealing non-invasive technique to sample pulmonary fluids. This hypothesis-generating study aims to (1) validate an ion chromatography method allowing the robust determination of different metabolism-related molecules(lactate, formate, acetate, propionate, butyrate, pyruvate, nitrite, nitrate)in EBC; (2) apply this method to EBC samples collected from workers exposed to quartz (a known inflammatory particle), to soapstone (a less inflammatory particle than quartz), as well as to controls. A multi-compound standard solution was used to determine the linearity range, detection limit, repeatability and bias from spiked EBC. The biological samples were injected without further treatment into an ion chromatograph with a conductivity detector. RTube® were used for field collection of EBC from 11 controls, 55 workers exposed to soapstone and 12 volunteers exposed to quartz dust. The analytical method used proved to be adequate for quantifying eight anions in EBC samples. Its sub-micromolar detection limits and repeatability, combined with a very simple sample preparation, allowed an easy and fast quantification of different glycolysis or nitrosative stress metabolites. Using multivariate discriminant analysis to maximize differences between groups, we observed a different pattern of anions with a higher formate/acetate ratio in the EBC samples for quartz exposed workers compared to the two other groups. We hypothesize that a modification of the metabolic signature could be induced by exposure to inflammatory particles like quartz and might be observed in the EBC via a change in the formate/acetate ratio.Item Estudo de série histórica de casos de tuberculose entre 1999 e 2015, em Ouro Preto, Minas Gerais, Brasil.(2021) Silva, Danilo Jorge da; Abreu, Frederico Prado; Xavier, Luiz Eduardo de Freitas; Duarte, Luana Pimentel; Neves, Vinícius de Jesus Rodrigues; Souza, Anelise Andrade de; Dias, Samuel Ribeiro; Carneiro, Ana Paula Scalia; Bezerra, Olívia Maria de Paula AlvesA tuberculose, caracterizada pela Organização Mundial de Saúde (OMS) como emergência sanitária mundial, é uma doença de impacto global. Objetivo: Realizar série histórica de casos de tuberculose em um período de 17 anos em Ouro Preto, Minas Gerais, considerando a histórica relação da doença com a mineração. Método: Dados foram obtidos em sistema próprio de registros do município, por busca ativa em prontuários médicos e comparados à quantidade de casos notificados no Sistema de Informação de Agravos de Notificação (SINAN). Para análises de tendência, foram utilizados modelos de regressão polinomial para séries históricas. Resultados: Idade média dos casos foi 40,3 ± 16,4 anos. Homens apresentaram 2,23 vezes mais casos e chance 2,07 vezes maior para desfechos negativos. A forma mais observada foi pulmonar (84%), e sorologia para HIV foi realizada em apenas 16,3% dos registros. Principal desfecho observado foi cura (70%), e desfechos negativos totalizaram 20,2% dos registros. Taxa de incidência média foi 29,76 e 16,23 casos/100 mil habitantes na área municipal e distrital, respectivamente. Conclusão: Apesar da relação histórica entre mineração e tuberculose no município, observa-se que este ainda apresenta preocupantes vulnerabilidades em relação à vigilância da doença. Análise de série temporal sugeriu declínio na proporção de casos curados entre 2009 e 2015.Item Inflammatory and oxidative stress biomarkers induced by silica exposure in crystal craftsmen.(2020) Carneiro, Ana Paula Scalia; Algranti, Eduardo; Chérot-Kornobis, Nathalie; Bezerra, Frank Silva; Bon, Ana Maria Tibiriça; Braz, Nayara Felicidade Tomaz; Souza, Débora Maria Soares de; Costa, Guilherme de Paula; Bussacos, Marco Antônio; Bezerra, Olívia Maria de Paula AlvesBackground: Identification of biomarkers associated with the diagnosis and prognosis of silicosis would be highly advantageous in the clinical setting. The aim of this study is to evaluate inflammatory and oxidative stress biomarkers in subjects exposed to silica. Methods: A cross‐sectional study of crystal craftsmen currently (n = 34) or formerly (n = 35) exposed and a group of nonexposed subjects (n = 12) was performed. Personal respirable dust samples were collected. Plasma inflammatory mediators (bone morphogenetic protein‐ BMP2 and chemokines CXCL16, and CCL5), oxidative stress enzymes (thiobarbituric acid reactive substances [TBARs] and superoxide dismutase [SOD]), and nitrite (NO2 − ) were analyzed in parallel with nitric oxide in exhaled breath (FeNO). Results: Being currently or formerly exposed to silica was related to increased levels of CXCL16 and TBARs. Currently, exposed subjects showed decreased levels of SOD. Thirty‐seven craftsmen with silicosis (26 formerly and 11 currently exposed) showed higher levels of CXCL16, which was positively associated with the radiological severity of silicosis. Compared with the nonexposed, subjects with silicosis had higher levels of TBARs and those with complicated silicosis had lower levels of SOD. In multivariate analysis, higher levels of CXCL16 were associated with exposure status and radiological severity of silicosis. Smoking was not a confounder. FeNO did not distinguish between the exposure status and the presence of silicosis. Conclusion: CXCL16 emerged as a potential biomarker that could distinguish both silica exposure and silicosis. TBARs were elevated in exposed individuals. However, their clinical applications demand further investigation in follow‐up studies of representative samples.Item Method validation of nanoparticle tracking analysis to measure pulmonary nanoparticle content : the size distribution in exhaled breath condensate depends on occupational exposure.(2017) Sauvain, Jean-Jacques; Suárez, Gina Wirz; Edmé, Jean Louis; Bezerra, Olívia Maria de Paula Alves; Silveira, Keller Guimarães; Amaral, Lênio S.; Carneiro, Ana Paula Scalia; Chérot-Kornobis, Nathalie; Sobaszek, Annie; Hulo, SébastienAparticle exposure assessment based on the dose deposited in the lungs would be the gold standard for the evaluation of any resulting health effects. Measuring particles in exhaled breath condensate (EBC)—a matrix containing water and airway lining fluid—could help to evaluate particle retention in the lungs. This study aimed to (1) validate a nanoparticle tracking analysis (NTA) method for determining the particle number concentration and their hydrodynamic size distribution in EBC, and (2) apply this method to EBC collected from workers exposed to soapstone (n=55) or quartz dust (n=12) and controls (n=11). A standard latex bead solution was used to determine the linear range, limit of detection (LOD), repeatability (coefficient of variation,CV), and bias in spiked EBC. An LM10 NanoSight instrument withNTAversion 3.1 software was used for measurement. RTubes® were used for field collection of EBC. The repeatability obtained for aD50 size distribution in EBC showed less than8%variability, with a bias<7%. The particle concentration was linear in the range 2.5×108 particles ml−1 with aLODof 4×106 particles ml−1. A recovery of 117±20% at 6.2×107 particles ml−1 was obtained with aCV<10% and a bias<20%. EBC from workers exposed to quartz, who experienced the largest exposure to silica particles, consistently exhibited a statistically significant (p<0.01) higher concentration of particles in their EBC, with a size distribution shift towards larger values than the other groups. Results showed that theNTAtechnique performed well for characterizing the size distribution and concentrations of particles in EBC. The technique needs to be corroborated with a larger population of workers.Item Recombinant phages for Spodoptera frugiperda control : new perspectives to tackle an economic problem.(2020) Nunes, Sarah Braga Rodrigues; Mota, Sara Teixeira Soares; Guerra, Joyce Ferreira da Costa; Moura, Joyce Dorneles; Carneiro, Ana Paula Scalia; Vecchi, Lara; Carvalho, Mayara Medeiros de Freitas; Lima, Wanderson Geraldo de; Araújo, Thaíse Gonçalves deThe Spodoptera frugiperda is an important pest responsible for large productivity losses of maize. Insecticidal proteins from Bacillus thuringiensis have promoted substantial advances for this pest control. However, since the occurrence of resistant insects is challenging this technology, it has become crucial to develop new effective products by using innovative techniques that allow the identification of new molecules. The aim of this study was to select recombinant phages expressing exogenous peptides binders that may act against S. frugiperda. By using Phage Display technology, seven recombinant phages were successfully selected and bound to intestinal proteins. The SfF3 phage demonstrated similarity to the ABC transporter subfamily C2. In in vivo assay, wild-type phage reduced the toxicity of the B. thuringiensis toxin, and SfF3 phage has rescued the mortality of S. frugiperda neonates when used in combination with the toxin. Our innovative study validated the reliability of Phage Display technology as an agribiotechnological approach for pest control, expanding the options to identify new molecules with bioinsecticides activities.Item Silica exposure and disease in semi-precious stone craftsmen, Minas Gerais, Brazil.(2017) Carneiro, Ana Paula Scalia; Braz, Nayara Felicidade Tomaz; Algranti, Eduardo; Bezerra, Olívia Maria de Paula Alves; Araújo, Natália Pereira da Silva; Amaral, Lênio S.; Edmé, Jean Louis; Sobaszek, Annie; Chérot-Kornobis, NathalieBackground Brazil is an exporter of precious stones and craftsmen often work in poor conditions. We assessed silica-related diseases among crystal craftsmen and the complexity of its control. Methods Case-series including 118 subjects evaluated from 2006 to 2015, based on medical interviews, chest X-rays, spirometry, and respirable silica samples. Results Median age and length of exposure were 32 and 13 years, respectively. Silicosis, with 1/0 as a threshold, was diagnosed radiologically in 57 individuals (48.3%). Respirable silica concentrations were 0.9–29.3 times greater than the Brazilian occupational exposure limit. A Receiver Operating Characteristic (ROC) curve with the same diagnosis threshold showed best discrimination at a cut point of 12.5 years of exposure, corresponding to 4.85 mg-y/m3 of cumulative silica exposure. There was a significant decline in FEV1 across radiological and cumulative silica exposure categories. Eleven individuals (9.3%) had mycobacterial diseases at baseline or follow-up. Conclusion Crystal craftsmen continue to suffer from silicosis, lung function impairment, comorbidity, and death due to silicosis. To date collective protection in some work sheds has not diminished silica levels. Long-term follow-up is needed to evaluate further improvements in preventive measures.Item Talcose entre artesãos em pedra-sabão em uma localidade rural do Município de Ouro Preto, Minas Gerais, Brasil.(2003) Bezerra, Olívia Maria de Paula Alves; Dias, Elizabeth Costa; Galvão, Márcio Antônio Moreira; Carneiro, Ana Paula ScaliaA talcose é uma pneumoconiose ainda pouco estudada em nosso meio. Sua ocorrência foi investigada no Distrito de Mata dos Palmitos, Ouro Preto, Minas Gerais, Brasil, entre artesãos em pedra-sabão, que trabalham em produção de base familiar, de caráter informal. Na localidade, vivem cerca de 180 habitantes, dos quais 108 se ocupam com o artesanato em pedra-sabão e 15 alternam a produção de peças artesanais com as atividades nas minas. Foram realizados exames clínicos, enfatizando a história ocupacional; questionário padronizado de sintomas respiratórios; radiografia de tórax (padrão OIT) e espirometria. Observou-se baixa prevalência de queixas respiratórias, e a dispnéia foi o sintoma mais freqüente. A ocorrência de bronquite crônica foi identificada em 12 trabalhadores adultos. O exame radiológico de tórax evidenciou pequenas opacidades pulmonares em cinco trabalhadores, e 11 foram considerados suspeitos. Um trabalhador apresentou espessamento pleural em placa. Alterações na espirometria foram observadas em sete trabalhadores. O estudo da composição da poeira revelou a presença de fibras respiráveis de asbesto do grupo dos anfibólios (tremolita-actinolita). Esses resultados sugerem a ocorrência de talcoasbestose entre os artesãos em pedra-sabão.Item Talcosis in soapstone artisans : high-resolution CT findings in 12 patients.(2014) Faria, Henrique Pereira; Veiga, Alessandro de Souza; Teixeira, Luísa Coutinho; Bezerra, Olívia Maria de Paula Alves; Carneiro, Ana Paula Scalia; Ferreira, C. S.; Marchiori, ElenaAIM: To describe the high-resolution computed tomography (HRCT) features of pneumoconiosis observed in soapstone artisans. MATERIALS AND METHODS: The present study included 12 soapstone artisans with chest radiography abnormalities consistent with the diagnosis of pneumoconiosis, in accordance with the International Labour Office classification. All patients had undergone HRCT, and the images were retrospectively analysed by two chest radiologists, who reached decisions in consensus. RESULTS: All patients presented with interlobular septal thickening. Small centrilobular nodules (75%) and ground-glass opacities (67%) were also common findings. The distributions of abnormalities were predominantly diffuse. No pleural abnormality was found. CONCLUSION: The HRCT abnormalities observed in this group of soapstone artisans are similar to those of pure talc pneumoconiosis.Item Talcosis related to exposure index among soapstone handicraft workers in Ouro Preto, Minas Gerais, Brazil.(2016) Neves, A.; Carneiro, Ana Paula Scalia; Bezerra, Olívia Maria de Paula Alves; Rocca, P.Introdução Apesar da grande utilização de pedra-sabão na produção de artesanatos, especialmente na região de Ouro Preto-MG, existem poucas publicações sobre talcose no Brasil. Objetivo Relacionar a ocorrência de talcose com níveis de exposição à poeira de pedra-sabão entre artesãos de Ouro Preto. Metodologia Foram reanalisadas radiografias e espirometrias de 74 artesãos de um estudo original, realizado há alguns anos. Foi elaborado um índice de exposição à poeira de pedra sabão a partir da revisão dos registros de avaliações dos ambientes de trabalho realizadas por ocasião do estudo original. Ajustou-se um modelo de regressão logística para verificar a associação da exposição com suspeita ou presença de pneumoconiose. Resultados A idade de início da exposição ocupacional e o tempo de exposição tiveram mediana de 15,5 e 15 anos, respetivamente. Quatro pacientes apresentaram imagens compatíveis com pneumoconiose, oito apresentaram suspeita radiológica da doença e um apresentava placa pleural. Houve associação do tempo e do índice de exposição com a presença ou suspeita radiológica de pneumoconiose. Apenas 34 espirometrias preencheram critérios técnicos para análise e a capacidade vital forçada apresentou diferença significativa entre o grupo controlo e aquele alterado/suspeito de pneumoconiose. Conclusão Confirmou-se a conhecida relação dose-resposta da exposição a poeiras de pedra-sabão associada à pneumoconiose e o adoecimento em população jovem, sem acesso a acompanhamento médico regular e desprotegida do ponto de vista previdenciário, por estar inserida no mercado informal de trabalho.