Navegando por Autor "Cabral, Daniela Bastos"
Agora exibindo 1 - 1 de 1
Resultados por página
Opções de Ordenação
Item Yeast genes YOL002C and Sul1 are involved in neomycine resistance.(2001) Castro, Ieso de Miranda; Cabral, Daniela Bastos; Trópia, Maria José Magalhães; Barnabé, Fabiana Maria de Almeida; Brandão, Rogélio LopesIn previous studies we suggested the importance of the control of plasma membrane H+-ATPase by a phosphatidylinositol-like pathway for cellular proton extrusion in Saccharomyces cerevisiae (Brandão et al. 1994; Coccetti et al. 1998). The observations that provided the model above include the inhibition of the glucose-induced activation of the plasma membrane H+-ATPase as well as the inhibition of the glucose-induced external acidification by neomycin, a known inhibitor of the phosphatidylinositol turnover in eukaryotic cells. In this work, using two libraries, we isolated two yeast clones that were able to prevent the inhibition of glucose-induced activation of the H+-ATPase by neomycin. We show that the YOL002C gene, which encodes a protein of unknown function, and the SUL1 gene, which is a sulphate transporter belonging to the major facilitator superfamily, suppress growth inhibition by neomycin. However, they are not required for glucose-induced activation of the plasma membrane H+-ATPase. The resistance of the clones to neomycin is probably related to the level and/or activity of proteins functioning as drug extrusion pumps.