Characterization of export receptor exportins (XPOs) in the parasite Schistosoma mansoni.
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2013
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Several proteins and different species of RNA that
are produced in the nucleus are exported through the nuclear
pore complexes, which require a family of conserved nuclear
export receptors called exportins (XPOs). It has been reported
that the XPOs (XPO1, XPO5, and XPOT) are directly involved
in the transport processes of noncoding RNAs from the
nucleus to the cytoplasm and/or from cytoplasm to the nucleus.
All three genes are present in fungi, plants, and deuterostome
metazoans. However, protostome metazoan species
lack one of the three genes across evolution. In this report,
we have demonstrated that all three XPO proteins are present
in the parasite protostome Schistosoma mansoni . As this
parasite has a complex life cycle presenting several stages in
different hosts and environments, implying a differential gene
regulation, we proposed a genomic analysis of XPOs to validate
their annotation. The results showed the conservation of
exportin family members and gene duplication events in S.
mansoni .We performed quantitative RT-PCR, which revealed
an upregulation of SmXPO1 in 24 h schistosomula (sixfold
when compared with cercariae), and similar transcription
levels were observed for SmXPO5 and SmXPOT in all the
analyzed stages. These three XPO proteins have been identified
for the first time in the protostome clade, which suggests a
higher complexity in RNA transport in the parasite S.
mansoni. Taken together, these results suggest that RNA
transport by exportins might control cellular processes during
cercariae, schistosomula, and adult worm development.
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ABREU, F. C. P. de et al. Characterization of export receptor exportins (XPOs) in the parasite Schistosoma mansoni. Parasitology Research, v. 1, p. 1-9, 2013. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-013-3606-x>. Acesso em: 23 fev. 2017.