Effects of specific treatment on parasitological and histopathological parameters in mice infected with different Trypanosoma cruzi clonal genotypes.
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2004
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The goal of this study was to verify the effect of specific treatment on parasitological and histopathological
parameters in mice experimentally infected with different Trypanosoma cruzi clonal genotypes. Twenty
cloned stocks were selected, representative of the whole phylogenetic diversity of the protozoan and
belonging to the clonal genotypes 19 and 20 (T. cruzi I) and 39 and 32 (T. cruzi II). The stocks were inoculated
in 40 BALB/c mice divided into four groups: (i) treated with benznidazole, (ii) treated with itraconazole and
(iii and iv) untreated control groups (NT) for each drug, respectively. Seven parameters related to parasitaemia
curves and histopathological lesions were analysed. Four during the acute phase (AP) and three during both
the AP and chronic phase (CP) of infection. Statistical comparison between benznidazole-treated and NT
groups for the biological parameters showed significant differences for all genotypes. Benznidazole treatment
led to lower patent period, maximum of parasitaemia, day of maximum parasitaemia and area under the
parasitaemia curve for all genotypes analysed. Percentage of positive haemoculture during AP and CP was
lower for genotypes 19 and 32. Tissue parasitism (TP) and inflammatory process (IP) during AP were lower
for genotypes 19 and 32, respectively. In general, itraconazole treatment induced a smaller reduction in
these same parameters between treated and NT animals in relation to benznidazole treatment. Our results
indicate that phylogenetic divergence among T. cruzi clonal genotypes must be taken in account in chemotherapy
and studies dealing with all aspects of the parasite and the disease.
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Experimental chemotherapy, Benznidazole, Itraconazole, Genetic groups
Citação
ORNELAS, M. J. de et al. Effects of specific treatment on parasitological and histopathological parameters in mice infected with different Trypanosoma cruzi clonal genotypes. Journal Of Antimicrobial Chemotherapy, v. 53, n. 6, p.1045-1053, 2004. Disponível em: <http://jac.oxfordjournals.org/content/53/6/1045.long>. Acesso em: 10 jan. 2017.