Histopathological features, parasite density and cell phenotype of the popliteal lymph node in canine visceral leishmaniasis.
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2008
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While enlargement of popliteal lymph nodes (LN) is frequently described in canine visceral leishmaniasis (CVL), there are few
histopathologic studies of lymph nodes during this chronic immunopathological condition.Besides a detailed histopathologic analysis,
we have characterized the parasite load andmajor immunophenotypic features of theLNin Leishmania (Leishmania) chagasi-infected
dogs. Our major histopathological findings highlight that hypertrophy/hyperplasia of LN cortical and medullary zones was the
principal characteristic observed in asymptomatic dogs (AD), whereas atrophy of LN cortical zone was predominant in symptomatic
animals (SD). The LN parasite density detected by anti-Leishmania immunohistochemical assay or expressed as Leishman Donovan
Units was also highly correlated with the skin parasitism, the most reliable parameter to decode the clinical status of CVL. The major
LN immunophenotypic changes during ongoing CVL were an increased frequency of T-lymphocytes, particularly CD8+ T-cells, upregulation
of MHC-II expression by lymphocytes and decreased levels of CD21+ B-cells. Our findings further demonstrated that
changes in the LNB-lymphocyte compartment exhibited a negative correlation with the skin parasite load. Conversely, we also showed
evidence for a positive association between skin parasitismandLNT-cell-mediated immunity, suggesting thatT-cells, especiallyCD8+
lymphocytes, may have a Type-2 immunological profile in this lymphoid tissue in response to CVL.
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Leishmania chagasi, Parasitism, Lymphocyte subsets, Flow cytometry
Citação
GIUNCHETTI, R. C. et al. Histopathological features, parasite density and cell phenotype of the popliteal lymph node in canine visceral leishmaniasis. Veterinary Immunology And Immunopathology, v. 121, p.23-33, 2008. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016524270700267X>. Acesso em: 10 out. 2016.