High-resolution mass spectrometry identification and characterization of flavonoids from Fridericia chica leaves extract with anti-arbovirus activity.
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Data
2022
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Resumo
Plant extracts are complex mixtures that are difficult to characterize, and mass spectrometry
is one of the main techniques currently used in dereplication processes. Fridericia chica is a species with
medicinal uses in Latin American countries, used in the treatment of inflammatory and infectious
diseases. Extracts of this plant species are characterized by the presence of anthocyanidins. In this
study, using high-resolution mass spectrometry coupled with liquid chromatography, it was possible
to determine the molecular formula of thirty-nine flavonoids. Fragmentation analysis, ultraviolet
spectrum and nuclear magnetic resonance data allowed the partial characterization of the structures
of these compounds. The spectral dataset allowed the identification of a series of flavones in addition
to the desoxyanthocyanidins common in extracts of the species. The occurrence of some of the
proposed structures is uncommon in extracts of species of the Bignoniaceae family, and they are
reported for the first time in the extract of this species. Quantitative analyses of total flavonoids
confirmed the high content of these constituents in the species, with 4.09 ± 0.34 mg/g of dry plant
material. The extract under study showed low in vitro cytotoxicity with CC50 ≥ 296.7 ± 1.4 μg/mL
for Vero, LLC-MK2 and MRC-5 cell lines. In antiviral activity assays, inhibition of the cytopathic
effects of Dengue, Zika and Mayaro viruses was observed, with EC50 values ranging between 30.1
and 40.9 μg/mL. The best result was observed against the Mayaro virus, with an EC50 of 30.1 μg/mL.
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Arrabidaea chica, Bignoniaceae, Anti-dengue activity
Citação
CRUZ, A. F. G. da et al. High-resolution mass spectrometry identification and characterization of flavonoids from Fridericia chica leaves extract with anti-arbovirus activity. Molecules, v. 27, n. 18, artigo 6043, 2022. Disponível em: <https://www.mdpi.com/1420-3049/27/18/6043>. Acesso em: 01 ago. 2023.