Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata.
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2019
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BACKGROUND Biomphalaria glabrata is the major species used for the study of schistosomiasis-related parasite-host
relationships, and understanding its gene regulation may aid in this endeavor. The ubiquitin-proteasome system (UPS) performs
post-translational regulation in order to maintain cellular protein homeostasis and is related to several mechanisms, including
immune responses.
OBJECTIVE The aims of this work were to identify and characterise the putative genes and proteins involved in UPS using
bioinformatic tools and also their expression on different tissues of B. glabrata.
METHODS The putative genes and proteins of UPS in B. glabrata were predicted using BLASTp and as queries reference proteins
from model organism. We characterised these putative proteins using PFAM and CDD software describing the conserved
domains and active sites. The phylogenetic analysis was performed using ClustalX2 and MEGA5.2. Expression evaluation was
performed from 12 snail tissues using RPKM.
FINDINGS 119 sequences involved in the UPS in B. glabrata were identified, which 86 have been related to the ubiquitination
pathway and 33 to proteasome. In addition, the conserved domains found were associated with the ubiquitin family, UQ_con,
HECT, U-box and proteasome. The main active sites were lysine and cysteine residues. Lysines are responsible and the starting
point for the formation of polyubiquitin chains, while the cysteine residues of the enzymes are responsible for binding to
ubiquitin. The phylogenetic analysis showed an organised distribution between the organisms and the clades of the sequences,
corresponding to the tree of life of the animals, for all groups of sequences analysed. The ubiquitin sequence was the only one
with a high expression profile found in all libraries, inferring its wide range of performance.
MAIN CONCLUSIONS Our results show the presence, conservation and expression profile of the UPS in this mollusk, providing
a basis and new knowledge for other studies involving this system. Due to the importance of the UPS and B. glabrata, this work
may influence the search for new methodologies for the control of schistosomiasis.
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Snail, Ubiquitin-proteasome system - UPS, Signaling pathway, Bioinformatics, Schistosomiasis
Citação
PORTILHO, L. G. et al. Genome-wide identification, characterisation and expression profiling of the ubiquitin-proteasome genes in Biomphalaria glabrata. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, 2019. Disponível em: <https://www.scielo.br/j/mioc/a/fjWr9tGRcjhgtwPMxpLNQtK/?lang=en>. Acesso em: 11 out. 2022.