Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/handle/123456789/9623
Título: Validation of a new high-throughput method to determine urinary S-phenylmercapturic acid using low-temperature partitioning extraction and ultra high performance liquid chromatography–mass spectrometry.
Autor(es): Gomes, Rafaela de Paiva
Pena, Camila Bárbara
Rezende, Jennifer
Coutrim, Maurício Xavier
Afonso, Robson José de Cássia Franco
Palavras-chave: Benzene
Liquid chromatography
Low-temperature partition extraction
Data do documento: 2017
Referência: GOMES, R. de P. et al. Validation of a new high-throughput method to determine urinary S-phenylmercapturic acid using low-temperature partitioning extraction and ultra high performance liquid chromatography–mass spectrometry. Journal Of Separation Science, v. 40, p. 550-557, 2017. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1002/jssc.201600540/epdf>. Acesso em: 15 set. 2017.
Resumo: A new highly sensitive and environmentally friendly analytical method, using low-temperature partition extraction and ultra-high-performance liquid chromatography with tandem mass spectrometry, without the use of a labeled analyte, was developed and validated to determine and quantify urinary Sphenylmercapturic acid in urine samples. TheWorld Health Organization, in its guidelines for air quality in Europe, recognizes that benzene is carcinogenic to humans and there is no safe level of exposure. Urinary S-phenylmercapturic acid is a sensitive and specific biological marker of exposure to benzene. The new analytical method, extraction, and analysis, were linear in the working range between 0.1 and 200.0 μg/L, precise (relative standard deviation lower than 6.0%), accurate (97.0–105.0%), and sensitive. The method’s limits of detection and quantification were 0.02 and 0.084 μg/L, respectively. The recovery with the lowtemperature partition extraction was 96.1%, with relative standard deviation less than 3.8%. The method is simple, accurate, and reproducible, and has been successfully applied in the evaluation of nonoccupational exposure to benzene, by urinary S-phenylmercapturic acid in urine samples.
URI: http://www.repositorio.ufop.br/handle/123456789/9623
Link para o artigo: http://onlinelibrary.wiley.com/doi/10.1002/jssc.201600540/epdf
ISSN: 16159314
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