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http://www.repositorio.ufop.br/jspui/handle/123456789/9186
Título: | Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice. |
Autor(es): | Bandeira, Ana Carla Balthar Silva, Talita Prato da Araújo, Glaucy Rodrigues de Araújo, Carolina Morais Silva, Rafaella Cecília da Lima, Wanderson Geraldo de Bezerra, Frank Silva Costa, Daniela Caldeira |
Palavras-chave: | Hepatotoxicity Oxidative stress |
Data do documento: | 2017 |
Referência: | BANDEIRA, A. C. B. et al. Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice. Chemico-Biological Interactions, v. 263, p. 7-17, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0009279716307219>. cesso em: 15 set. 2017 |
Resumo: | Our aim was to investigate the antioxidant potential of lycopene in different experimental liver models: in vitro, to evaluate the influence of lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway. The role of NADPH oxidase was evaluated by measuring its inhibition potential using an inhibitor of this enzyme. In the in vivo study, male C57BL/6 mice received lycopene (10 or 100 mg/kg by oral gavage) and 1 h later, acetaminophen (APAP) (500 mg/kg) was administrated. Lycopene decreased ROS production in SK-Hep- 1 cells through inhibition of NADPH oxidase, brought about in the PKC pathway. Lycopene improved hepatotoxicity acting as an antioxidant, reduced GSSG and regulated tGSH and CAT levels, reduced oxidative damage primarily by decreasing protein carbonylation, promoted the downregulation of MMP- 2 and reduced areas of necrosis improving the general appearance of the lesion in C57BL/6 mice. Lycopene is a natural compound that was able to inhibit the production of ROS in vitro and mitigate the damage caused by APAP overdose in vivo. |
URI: | http://www.repositorio.ufop.br/handle/123456789/9186 |
Link para o artigo: | http://www.sciencedirect.com/science/article/pii/S0009279716307219 |
DOI: | https://doi.org/10.1016/j.cbi.2016.12.011 |
ISSN: | 0009-2797 |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_LycopeneInhibitsreative.pdf Restricted Access | 1,99 MB | Adobe PDF | Visualizar/Abrir |
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