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Título: | Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni. |
Autor(es): | Machado, Carla Botelho Cabral, Fernanda Janku Soares, Cláudia Sossai Moreira, Érika Bueno de Carvalho Morais, Enyara Rezende Magalhães, Lizandra Guidi Gomes, Matheus de Souza Cota, Renata Guerra de Sá Rosa, José César Ruller, R. Ward, R. J. Rodrigues, Vanderlei |
Data do documento: | 2010 |
Referência: | MACHADO, C. B. et al. Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni. Parasitology Research, v. 107, p. 1163-1171, 2010. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-010-1984-x>. Acesso em: 23 fev. 2017. |
Resumo: | The proline-rich inhibitor of 31 kDa (PI31) is highly conserved through metazoan evolution, and its activity in the proteasome inhibition is well-established although the precise mechanism of inhibition is unclear. The coding DNA sequence of Schistosoma mansoni PI31 (SmPI31) was cloned, and the recombinant protein was expressed in bacterial system. The correct amino acid sequence was confirmed by mass spectrometry and circular dichroism suggests that SmPI31 contains both α-helix and non-structured regions. Inhibition assays, using the SucLeu-Leu-Val-Tyr-4-MCA substrate for proteasome degradation, showed that the S. mansoni proteasome may be regulated by the inhibitory activity of SmPI31. A gene expression assay using qRT-PCR at various stages during the S. mansoni life cycle has shown that SmPI31 transcripts are expressed in all studied stages, suggesting that PI31 plays an important role during the developmental processes of the parasite. In this study first evidence is presented that PI31 has a conserved structure and plays a role as proteasome inhibitor in adult worms and it is expressed through life cycle. |
URI: | http://www.repositorio.ufop.br/handle/123456789/8955 |
Link para o artigo: | https://link.springer.com/article/10.1007%2Fs00436-010-1984-x |
DOI: | https://doi.org/10.1007/s00436-010-1984-x |
ISSN: | 1432-1955 |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
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ARTIGO_CharacterizationmRNAExpression.pdf Restricted Access | 557,41 kB | Adobe PDF | Visualizar/Abrir |
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