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http://www.repositorio.ufop.br/jspui/handle/123456789/8136
Título: | Plasma membrane proteomes of differentially matured dendritic cells identified by LC MS/MS combined with iTRAQ labelling. |
Autor(es): | Bernard, Stéphanie Ferret Borges, William de Castro Dowle, Adam A. Sanin, David E. Cook, Peter C. Turner, Joseph D. MacDonald, Andrew S. Thomas, Jerry R. Mountford, Adrian P. |
Palavras-chave: | Parasitic helminth |
Data do documento: | 2011 |
Referência: | FERRET-BERNARD, S. et al. Plasma membrane proteomes of differentially matured dendritic cells identified by LC MS/MS combined with iTRAQ labelling. Journal of Proteomics, v. 75, p. 938-948, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S1874391911004969>. Acesso em: 20 mar. 2017. |
Resumo: | Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear whatmolecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC–MS/MS to quantify changes in the proteomes. Analysis was performed on triplicate biological samples and changes verified by flow cytometry. MHC class II molecules and CD29 were up-regulated in pro-Th1 DCs whilst CD18 and CD44 were upregulated in pro-Th2 DCs. One of the most down-regulated molecules in pro-Th1 DCs was YM-1 whilst the greatest decrease in pro-Th2 DCs was NAP-22. Other molecules upregulated in pro-Th2 DC compared to pro-Th1 DCs included some potentially involved in protein folding during antigen processing (clathrin and Rab-7), whilst other non-membrane proteins such as enzymes/transporters related to cell metabolism(malate dehydrogenase, pyruvate kinase, and ATPase Na+/K+) were also recorded. This suggests that pro-Th2 DCs are more metabolically active while pro-Th1 DCs have a mature ‘end state’. Overall, althoughseveralmolecules were preferentially expressed on pro-Th2 DCs, our proteomics data support the view of a ‘limited maturation’ of pro-Th2 DCs compared to pro-Th1 DCs. |
URI: | http://www.repositorio.ufop.br/handle/123456789/8136 |
DOI: | https://doi.org/10.1016/j.jprot.2011.10.010 |
ISSN: | 1874-3919 |
Licença: | Este artigo está sob licença Creative Commons que permite copiar, distribuir e transmitir o trabalho desde que sejam citados o autor e o licenciante. Fonte: o próprio artigo. |
Aparece nas coleções: | DECBI - Artigos publicados em periódicos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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ARTIGO_PlasmaMemblaneProteomes.pdf | 1,88 MB | Adobe PDF | Visualizar/Abrir |
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