Protein-energy malnutrition decreases immune response to Leishmania chagasi vaccine in BALB/c mice.
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2009
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Resumo
Protein-energy malnutrition and visceral leishmaniasis are
important problems of public health affecting millions of people
worldwide. Vaccine efficacy depends on the ability of individuals
to mount an appropriate immune response and may be
inadequate in malnourished persons. In this study, we used a
mouse model to verify the effect of combined protein, iron and
zinc deficiency in the response to Leishmania chagasi antigen
vaccine. BALB/c mice were fed with a low-protein (3% casein),
iron- and zinc-deficient diet or control diet (14% casein and
sufficient in zinc and iron). After malnutrition establishment,
mice were vaccinated subcutaneously with L. chagasi Ag plus
saponin. After vaccination, mice were nutritionally repleted
and then all mice were challenged with L. chagasi promastigotes.
Four weeks later, liver and spleen parasite load was evaluated.
Our data show that vaccine caused a significant reduction in
parasite load in spleen and liver from mice fed with control
diet. However, splenic parasitism was increased in mice fed
with deficient diet and this diet caused a reduction in splenocyte
IFN-γ production in response to the vaccine in repleted
mice. These data suggest that malnutrition may alter immune
response to L. chagasi vaccine in BALB/c model of infection,
even after nutritional repletion.
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Nutritional repletion, Vaccination
Citação
MALAFAIA, G. et al. Protein-energy malnutrition decreases immune response to Leishmania chagasi vaccine in BALB/c mice. Parasite Immunology, v. 31, p. 41-49, 2009. Disponível em: <http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.2008.01069.x/pdf>. Acesso em: 20 jan. 2017.