Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge.
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Data
2005
Autores
Silva, Eduardo de Almeida Marques da
Coelho, Eduardo Antônio Ferraz
Gomes, Daniel Cláudio de Oliveira
Vilela, Márcia de Carvalho
Masioli, Cássio Zumerle
Tavares, Carlos Alberto Pereira
Fernandes, Ana Paula Salles Moura
Afonso, Luís Carlos Crocco
Rezende, Simone Aparecida
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Resumo
Acute visceral leishmaniasis is a progressive
disease caused by Leishmania chagasi in South America.
The acquisition of immunity following infection suggests
that vaccination is a feasible approach to protect against this
disease. Since Leishmania homologue of receptors for
activated C kinase (LACK) antigen is of particular interest
as a vaccine candidate because of the prominent role it plays
in the pathogenesis of experimental Leishmania major
infection, we evaluated the potential of a p36(LACK) DNA
vaccine in protecting BALB/c mice challenged with L.
chagasi. In this study, mice received intramuscular (i.m.) or
subcutaneous (s.c.) doses of LACK DNA vaccine. We
evaluated the production of vaccine-induced cytokines and
whether this immunization was able to reduce parasite load
in liver and spleen. We detected a significant production of
interferon gamma by splenocytes from i.m. vaccinated mice
in response to L. chagasi antigen and to rLACK protein.
However, we did not observe a reduction in parasite load
neither in liver nor in the spleen of vaccinated animals. The
lack of protection observed may be explained by a significant
production of IL-10 induced by the vaccine.
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Lack
Citação
SILVA, E. A. M. da et al. Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-gama production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge. Parasitology Research, v. 98, n.1, p. 67-74, 2005. Disponível em: <http://link.springer.com/article/10.1007%2Fs00436-005-0008-8>. Acesso em: 20 jan. 2017.