Use este identificador para citar ou linkar para este item: http://www.repositorio.ufop.br/handle/123456789/4916
Título: Choline oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices.
Autor(es): Prado, Marco Antonio Maximo
Santos, T. Moraes
Freitas, Renata Nascimento de
Silva, Marco Aurelio Romano
Gomez, Marcus Vinicius
Palavras-chave: Acetylcholine assay
Chemiluminescent procedure
Neurotransmitter release
Data do documento: 1990
Referência: PRADO, M. A. M. et al. Choline oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <http://ac.els-cdn.com/0165027090901102/1-s2.0-0165027090901102-main.pdf?_tid=fcde7f20-a1a7-11e4-80c7-00000aab0f27&acdnat=1421870540_90c45cb48c2da9786322c32e22671e6b>. Acesso em: 08 nov. 2014.
Resumo: A chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.
URI: http://www.repositorio.ufop.br/handle/123456789/4916
ISSN: 01650270
Licença: O Periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.
Aparece nas coleções:DENCS - Artigos publicados em periódicos

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