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Title: Performance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation.
Authors: Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Souza, Juliana Vitoriano de
Vital, Wendel Coura
Braga, Samuel Leôncio
Oliveira, Rodrigo Corrêa de
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
Lana, Marta de
Gontijo, Nelder de Figueiredo
Marques, Marcos José
Giunchetti, Rodolfo Cordeiro
Reis, Alexandre Barbosa
Keywords: Leishmania infantum
Issue Date: 2012
Citation: ROATT, B. M. Performance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation. Plos One, v. 7, n.11 p. e49780, 2012. Disponível em: <>. Acesso em: 14 ago. 2014.
Abstract: In the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during long periods after experimental infection in vaccine trials is still not fully understood. Herein, we investigated the immunogenicity and parasitological levels after intradermal challenge with Leishmania infantum plus salivary gland extract in dogs immunized with a vaccine composed of L. braziliensis antigens plus saponin as an adjuvant (LBSap vaccine). The LBSap vaccine elicited higher levels of total anti-Leishmania IgG as well as both IgG1 and IgG2. Furthermore, dogs vaccinated had increased levels of lymphocytes, particularly circulating B cells (CD21+) and both CD4+ and CD8+ T lymphocytes. LBSap also elicited an intense in vitro cell proliferation associated with higher levels of CD4+ T lymphocytes specific for vaccine soluble antigen and soluble lysate of L. infantum antigen even 885 days after experimental challenge. Furthermore, LBSap vaccinated dogs presented high IFN-c and low IL-10 and TGF-b1 expression in spleen with significant reduction of parasite load in this tissue. Overall, our results validate the potential of LBSap vaccine to protect against L. infantum experimental infection and strongly support further evaluation of efficiency of LBSap against CVL in natural infection conditions.
ISSN: 1932-6203
metadata.dc.rights.license: Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <>. Acesso em: 03 jan. 2017.
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