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Título: | Anti–zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. leaves. |
Autor(es): | Lima, Lienne D’Auria Reis, Adriana Cotta Cardoso Sousa, Jordano Augusto Carvalho Valente, Gabriel Mendonça Silva, Breno de Mello Magalhães, Cíntia Lopes de Brito Kohlhoff, Markus Teixeira, Luiz Fernando de Medeiros Brandão, Geraldo Célio |
Palavras-chave: | Cytotoxicity Phytochemistry |
Data do documento: | 2023 |
Referência: | LIMA, L. D. A. et al. Anti–zika virus activity and isolation of flavonoids from ethanol extracts of Curatella americana L. leaves. Molecules, v. 28, n. 6, artigo 2546, 2023. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0378517323005677>. Acesso em: 01 ago. 2023. |
Resumo: | The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian Cerrado, whose ethanolic extract showed significant in vitro anti–Zika virus activity by the MTT colorimetric method. Currently, there is no drug in clinical use specifically for the treatment of this virus; therefore, in this work, the antiviral and cytotoxic properties of the ethanolic extract, fractions, and compounds were evaluated. The ethanolic extract of the leaves showed no cytotoxicity for the human MRC-5 cell and was moderately cytotoxic for the Vero cell (CC50 161.5 ± 2.01 μg/mL). This extract inhibited the Zika virus multiplication cycle with an EC50 of 85.2 ± 1.65 μg/mL. This extract was fractionated using the liquid–liquid partition technique, and the ethyl acetate fraction showed significant activity against the Zika virus with an EC50 of 40.7 ± 2.33 μg/mL. From the ethyl acetate fraction, the flavonoids quercetin-3-O-hexosylgallate (1), quercetin-3-O-glucoside (2), and quercetin (5) were isolated, and in addition to these compounds, a mixture of quercetin-3- O-rhamnoside (3) and quercetin-3-O-arabinoside (4) was also obtained. The isolated compounds quercetin and quercetin-3-O-hexosylgallate inhibited the viral cytopathic effect at an EC50 of 18.6 ± 2.8 and 152.8 ± 2.0, respectively. Additionally, analyses by liquid chromatography coupled to a mass spectrometer allowed the identification of another 24 minor phenolic constituents present in the ethanolic extract and in the ethyl acetate fraction of this species. |
URI: | http://www.repositorio.ufop.br/jspui/handle/123456789/17554 |
DOI: | https://doi.org/10.3390/molecules28062546 |
ISSN: | 1420-3049 |
Licença: | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo. |
Aparece nas coleções: | DEFAR - Artigos publicados em periódicos |
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ARTIGO_AntiZikaVirus.pdf | 3,54 MB | Adobe PDF | Visualizar/Abrir |
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