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Title: A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis.
Authors: Ostolin, Thais Lopes Valentim Di Paschoale
Gusmão, Miriã Rodrigues
Mathias, Fernando Augusto Siqueira
Cardoso, Jamille Mirelle de Oliveira
Roatt, Bruno Mendes
Soares, Rodrigo Dian de Oliveira Aguiar
Ruiz, Jeronimo Conceição
Resende, Daniela de Melo
Brito, Rory Cristiane Fortes de
Reis, Alexandre Barbosa
Keywords: Visceral leishmaniasis
Leishmania infantum
Protozoan proteins
Polyepitope vaccine
Issue Date: 2022
Citation: OSTOLIN, T. L. V. D. P. et al. A specific Leishmania infantum polyepitope vaccine triggers Th1-type immune response and protects against experimental visceral leishmaniasis. Cellular Immunology, v. 380, artigo 104592, out. 2022. Disponível em: <>. Acesso em: 01 ago. 2023.
Abstract: The development of an immunogenic, effective, and safe vaccine is essential as an alternative for disease control. The present study aimed to evaluate the immunogenicity and efficacy potential of a polyepitope T-cell antigen candidate against visceral leishmaniasis in a murine model. BALB/c mice were immunized with three doses subcutaneously with Poly-T Leish alone or adjuvanted with Saponin plus Monophosphoryl lipid A, with 15-day intervals between doses, and challenged with 107 stationary-phase Leishmania infantum promastigotes via tail vein. Immunogenicity and parasitism in spleen and liver of immunized mice were evaluated 45 days postchallenge. Our results revealed that the immunization with Poly-T Leish and Poly-T Leish/SM increases the percentage of specific T (CD4+ and CD8+) lymphocytes proliferation in vitro after antigen-specific stimulation. Also, Poly-T Leish and Poly-T Leish/SM groups showed a high percentage of IFN-γ and TNF-α-producing T cells, meanwhile, the Poly-T Leish/SM group also showed an increased percentage of multifunctional T cells producing double and triple-positive (IFN-γ+TNF-α+IL-2+) cytokines. The immunization with Poly-T Leish or Poly-T Leish/ SM stimulated a decreased IL-4 and IL-10 compared to the Saline and adjuvant group. Poly-T Leish/SM immunized mice exhibit a noteworthy reduction in the parasite burden (spleen and liver) through real-time PCR (96%). Moreover, we observed higher nitrite secretion in 120-hour stimulated-culture supernatant using Griess method. We demonstrated that the Poly-T Leish/SM candidate was potentially immunogenic, providing enhancement of protective immune mechanisms, and conferred protection reducing parasitism. Our candidate was considered potential against visceral leishmaniasis, and eventually, could be tested in phase I and II clinical trials in dogs.
ISSN: 0008-8749
Appears in Collections:DEACL - Artigos publicados em periódicos

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