Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/17534
Title: Immunoprophylaxis using polypeptide chimera vaccines plus adjuvant system promote Th1 response controlling the spleen parasitism in hamster model of visceral leishmaniasis.
Authors: Gusmão, Miriã Rodrigues
Ostolin, Thais Lopes Valentim Di Paschoale
Carvalho, Lívia Mendes
Costa, Ana Flávia Pereira
Moreira, Gabriel José Lucas
Cardoso, Jamille Mirelle de Oliveira
Soares, Rodrigo Dian de Oliveira Aguiar
Reis, Alexandre Barbosa
Brito, Rory Cristiane Fortes de
Roatt, Bruno Mendes
Keywords: Leishmania infantum
Chimera vaccines
Immunoinformatic
Immunogenicity
Hamster
Issue Date: 2022
Citation: GUSMÃO, M. R. et al. Immunoprophylaxis using polypeptide chimera vaccines plus adjuvant system promote Th1 response controlling the spleen parasitism in hamster model of visceral leishmaniasis. Vaccine, v. 40, n. 37, p. 5494-5503, set. 2022. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0264410X22009616?via%3Dihub>. Acesso em: 01 ago. 2023.
Abstract: In recent years, several advances have been observed in vaccinology especially for neglected tropical diseases (NTDs). One of the tools employed is epitope prediction by immunoinformatic approaches that reduce the time and cost to develop a vaccine. In this scenario, immunoinformatics is being more often used to develop vaccines for NTDs, in particular visceral leishmaniasis (VL) which is proven not to have an effective vaccine yet. Based on that, in a previous study, two predicted T-cell multi-epitope chimera vaccines were experimentally validated in BALB/c mice to evaluate the immunogenicity, central and effector memory and protection against VL. Considering the results obtained in the mouse model, we assessed the immune response of these chimeras in Mesocricetus auratus hamster, which displays, experimentally, similar pathological status to human and dog VL disease. Our findings indicate that both chimeras lead to a dominant Th1 response profile, inducing a strong cellular response by increasing the production of IFN-c and TNF-a cytokines associated with a decrease in IL-10. Also, the chimeras reduced the spleen parasite load and the weight a correlation between protector immunological mechanisms and consistent reduction of the parasitic load was observed. Our results demonstrate that both chimeras were immunogenic and corroborate with findings in the mouse model. Therefore, we reinforce the use of the hamster as a pre-clinical model in vaccination trials for canine and human VL and the importance of immunoinformatic to identify epitopes to design vaccines for this important neglected disease.
URI: http://www.repositorio.ufop.br/jspui/handle/123456789/17534
metadata.dc.identifier.uri2: https://www.sciencedirect.com/science/article/pii/S0264410X22009616?via%3Dihub
metadata.dc.identifier.doi: https://doi.org/10.1016/j.vaccine.2022.08.005
ISSN: 0264-410X
Appears in Collections:DEACL - Artigos publicados em periódicos

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