Please use this identifier to cite or link to this item: http://www.repositorio.ufop.br/jspui/handle/123456789/13744
Title: In silico pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of Humulus lupulus leaves cultivated in Brazil.
Authors: Silva, Regislainy Gomes da
Almeida, Tamires Cunha
Reis, Adriana Cotta Cardoso
Vieira Filho, Sidney Augusto
Brandão, Geraldo Célio
Silva, Glenda Nicioli da
Sousa, Hildeberto Caldas de
Almeida, Vera Lúcia de
Lopes, Júlio César Dias
Souza, Gustavo Henrique Bianco de
Issue Date: 2020
Citation: SILVA, R. G. da. et al. In silico pharmacological prediction and cytotoxicity of flavonoids glycosides identified by UPLC-DAD-ESI-MS/MS in extracts of Humulus lupulus leaves cultivated in Brazil. Natural Product Research, ago. 2020. Disponível em: <https://www.tandfonline.com/doi/abs/10.1080/14786419.2020.1803308>. Acesso em: 12 maio 2021.
Abstract: Ethanolic (EB) extract and hexanic (SH) and hydromethanolic (SEM) sub-extracts of Humulus lupulus leaves were submitted to cytotoxicity evaluation and to phytochemical methods. The effect of EB and SEM on cellular cycle was evaluated by propidium iodide method and the phases were quantified through flow cytometry. The cytotoxicity assessment was done using T24 and MRC5 cells, with EB and SEM (25–1200 mg/mL). By means of UPLC-DADMS/MS data were identified the flavonoids astragaline, nicotiflorin, kaempferol-7-O-rutinoside, robinin, hyperin, rutin, quercetin-7-Orutinoside and manghaslin. EB (800 mg/mL) and SEM (1200 mg/mL) reduces the T24 cell viability. These extracts at 25 mg/mL stimulate the growth of MRC5 cells, evidencing a selective cytotoxicity. After 24 h of the treatment with extracts was not observed cycle arrest of T24 cells. The bioactivity prediction of the flavonoids was evaluated in silico through in house Active-IT software and PASSonline which indicated potential activity as antitumoral, cytotoxic, anti-inflammatory, antiparasitic, antimicrobial, antiviral and others.
URI: http://www.repositorio.ufop.br/jspui/handle/123456789/13744
metadata.dc.identifier.uri2: https://www.tandfonline.com/doi/abs/10.1080/14786419.2020.1803308
metadata.dc.identifier.doi: https://doi.org/10.1080/14786419.2020.1803308
ISSN: 1478-6427
Appears in Collections:DEBIO - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
ARTIGO_SilicoPharmacologicalPrediction.pdf
  Restricted Access
1,17 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.