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dc.contributor.authorAlmeida, Tamires Cunha-
dc.contributor.authorGuerra, Camila Chaves Coelho-
dc.contributor.authorAssis, Bárbara Letícia Gonçalves de-
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar-
dc.contributor.authorGarcia, Camila Carrião Machado-
dc.contributor.authorLima, Angélica Alves-
dc.contributor.authorSilva, Glenda Nicioli da-
dc.identifier.citationALMEIDA, T. C. et al. Antiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status. Environmental and Molecular Mutagenesis, v. 60, n. 8, p.740-751, maio 2019. Disponível em: <>. Acesso em: 10 fev. 2020.pt_BR
dc.description.abstractThe antitumor activity of resveratrol, a polyphenolic compound found mainly in grapes, has been studied in several types of cancer. In bladder cancer, its antiproliferative effects have already been demonstrated; however, its mechanism of action is not completely understood. The aim of this study was to evaluate resveratrol antitumor activity (12.5, 25, 50, 100, 150, 200, and 250 μM) and its possible mechanisms of action in bladder tumor cells with different TP53 gene status (RT4, grade 1, TP53 wild type; 5637-grade 2 and T24-grade 3, TP53 mutated). Cell proliferation, clonogenic survival, morphological changes, cell cycle progression, apoptosis rates, genotoxicity, global methylation, immunocytochemistry for p53 and PCNA and relative expression profiles of the AKT, mTOR, RASSF1A, HOXB3, SRC, PLK1, and DNMT1 were evaluated. Resveratrol decreased cell proliferation and induced DNA damage in all cell lines. Regarding the long-term effects, resveratrol reduced the number of colonies in all cell lines; however, TP53 wild type cells were more resistant. Increased rates of apoptosis were found in the TP53 wild type cells and this was accompanied by AKT, mTOR, and SRC downregulation. In addition, the resveratrol antiproliferative effects in wild type TP53 cells were accompanied by modulation of the DNMT1 gene. In the TP53 mutated cells, cell cycle arrest at S phase with PLK1 downregulation was observed. Additionally, there was modulation of the HOXB3/RASSF1A pathway and nuclear PCNA reduction in the highest-grade cells. In conclusion, resveratrol has antiproliferative activity in bladder tumor cells; however, the mechanisms of action are dependent on TP53 status.pt_BR
dc.titleAntiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Appears in Collections:DECBI - Artigos publicados em periódicos

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