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dc.contributor.authorRosário, Eliza Yoshie do-
dc.contributor.authorGenaro, Odair-
dc.contributor.authorSilva, João Carlos França da-
dc.contributor.authorCosta, Roberto Teodoro da-
dc.contributor.authorMayrink, Wilson-
dc.contributor.authorReis, Alexandre Barbosa-
dc.contributor.authorCarneiro, Mariângela-
dc.identifier.citationROSÁRIO, E. Y. do et al. Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis. Memórias do Instituto Oswaldo Cruz, v. 100, n. 2, p. 197-203, 2005. Disponível em: <>. Acesso em: 10 jul. 2012pt_BR
dc.description.abstractThe performances of ELISA assays with different antigen preparations, such as Leishmania amazonensis or L. chagasi lysates and the recombinant antigens rK-39 and rK-26, were compared using sera or eluates from dried blood collected on filter paper to detect anti-Leishmania antibodies in dogs from a visceral leishmaniasis-endemic area in Brazil. Of 115 IFAT-reactive dogs at 1:40 titre, 106 (92.2%) were positive in parasitological exams (skin and/or spleen). These animals were compared to healthy animals (n = 25), negative for IFAT at a titre of 1:40 and parasitological exams. The sensitivities of crude and recombinant antigens were similar and remarkably high for both sera and eluates (97-100%). Specificity was higher than 96% for sera and eluates for different antigens, except for L. chagasi antigen using eluates (88%). Concordance values among the tests were higher either for sera or eluates (J = 0.95-1.00). High concordances were observed between sera and eluates tested with different antigens (kappa = 0.93-0.97). Crude and recombinant antigens identified different clinical phases of canine leishmaniasis. These results show that eluates could be used in canine surveys to identify L. chagasi infection. Recombinant antigens added little when compared to crude antigen in identifying positive dogs. Cross-reactivity with other diseases whose distribution often overlaps VL-endemic areas is a limitation of crude antigen use however.pt_BR
dc.subjectCanine visceral leishmaniasispt_BR
dc.subjectLeishmania chagasipt_BR
dc.subjectRecombinant antigenspt_BR
dc.titleEvaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
dc.rights.licenseO periódico, Memórias do Instituto Oswaldo Cruz permite que o Repositório Institucional da Universidade Federal de Ouro Preto deposite uma cópia eletrônica dos artigos publicados por esse periódico em que ao menos um dos autores faça parte da comunidade cientifica da Universidade Federal de Ouro Preto. Fonte: Licença concedida por e-mail em 12 dez. 2013.-
Appears in Collections:DEACL - Artigos publicados em periódicos

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