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Title: Prolonged cardioprotective effect of pyridostigmine encapsulated in liposomes.
Authors: Vidal, Alessandra Teixeira
Guimarães, Homero Nogueira
Paula, Danielle Cristiane Correa de
Frezard, Frederic Jean Georges
Barcellos, Neila Marcia Silva
Guimarães, Andrea Grabe
Keywords: Electrocardiogram
Sympathetic stimulation
Issue Date: 2010
Citation: VIDAL, A. T. et al. Prolonged cardioprotective effect of pyridostigmine encapsulated in liposomes. Life Sciences, v. 86, n.1–2, p.17–23, jan. 2010. Disponível em: <>. Acesso em: 10 jul. 2012.
Abstract: Aims: The purpose of the present work was to investigate the ability of pyridostigmine encapsulated in longcirculating liposomes, to protect against ECG(electrocardiogram) alterations induced by sympathetic stimulation in rats. Main methods: The encapsulation of pyridostigmine was carried out by freeze–thaw and extrusion. Blood pressure and ECG (limb lead II) were monitored in anaesthetized male Wistar rats. The formulation containing pyridostigmine was intravenously administrated in 0.1, 0.3 and 1.0 mg/kg doses, and sympathetic stimulation was conducted by administration of 1 or 3 μg of noradrenaline (NA) after 1, 2, 4 or 6 h. The obtained cardiovascular parameters were compared to animals that received intravenous injection of pyridostigmine in free form or saline. Key findings: After saline, NA induced a significant increase in QT interval (22.3% after 3.0 μg). Previous administration of free pyridostigmine significantly prevented the increase of QT interval after sympathetic stimulation and the most prominent effect was observed after 1h for the dose of 0.3 mg/kg (6.8% after 3.0 μg of NA) and was no longer observed after 2 h of the treatment. On the other hand, the maximum effect of pyridostigmine in liposomal formulation preventing QT interval increasewas observed 2 h after treatment (9.7% after 3.0 μg of NA) and was still present until 6 h when 1 mg/kg was previous administrated. Significance: The results of the present study, beyond to confirm the cardioprotective action of pyridostigmine, suggest that liposomal pyridostigmine may be a potential therapeutic alternative to prevent cardiovascular disturbances resulting from sympathetic hyperactivity.
ISSN: 00243205
metadata.dc.rights.license: O Periódico Life Sciences concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 500791718.
Appears in Collections:DEFAR - Artigos publicados em periódicos

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